Aniseed - Construct

Construct

ID

CONS00001684

Name

p.REG.CirobuKH.C11:1684423-1684923_EboxMut>LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

The 500bp Tnl enhancer, located upstream (-836/-335bp) of its TSS, carries a mutated E-box sequence (CAGCTG to acGCgt, lowercase in the text). It drives very weak LacZ expression.

This construct is testing Regulatory Region

REG00001595: REG.CirobuKH.C11:1684423-1684923_EboxMut|TnI

Sequence

Show sequence

Length: 501

GTAGGTGCTTGTGACGTCATACTGacGCgtTTATCGCCTGAGCAGCAGTGTTCCCCGTGA
TTTTGTGCTTTGAATCAAATTTAAATATTACAAATATAAAAAATATATTCTGTTATACTT
AATCAAAGTAAAGTATTGGTATAAGCATATTTTCGTTAATTTAGTCCAAATCAACTATAG
TTTAAGCAGTTTCTAGACGTTTACATCAGTACGGCGGCAACACTCGTTCTATATTAAATC
CAGACAACCAGAACTTCTTCTTCGTTTAGTCCCTCGATGTTCTATTTAAGGGAGCTTACA
GCATCCATCCAACATCGGTAATCGAGAATCTGTTAACCTTGTTTAGTCACAGCGAAAGGT
TCATGCATTTAAGAACCCCTTGGTGAATTTAAAGAATTTTCTTGTAGAAAGGGACGCGTA
GTGGCGATAGTTACTATGGTTAACTATTAGCTTAGCTGAGTTTATCATGATTACTATTTT
TATTATTTAGATTTCTGAAGG

Reference(s)

Izzi et al. (2013). Functional studies of the Ciona intestinalis myogenic regulatory factor reveal conserved features of chordate myogenesis. Dev Biol 376(2):213-23

Show in situ data