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CONSTRUCT

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  1. Construct 'CONS00001672'

Construct

ID

CONS00001672

Name

p.Cirobu.REG.KhL96:471236-471527_Tbox5-6-mut>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

The 294bp notochord enhancer of Tbx2/4, carrying a mutation of its 5th and 6th T-box binding sites, was cloned upstream of the basal promoter of Forkhead and LacZ reporter gene. This construct is weakly active in mesenchyme and tail muscles.

This construct is testing Regulatory Region

REG00001582: Cirobu.REG.KhL96:471236-471527_Tbox5-6-mut|Tbx2/3

Sequence

Show sequence

Length: 292

GTGGACAGACAGGGTAGTTAAATACGTTACAAAGTTCATTTGCCACCCAGCACAGTATTG
TACTTTATGACACTTGTGTTTATTCGTATAGTGGGACTAGTTATAAAATAAAACGGTTTA
TTCTCTGACACGTAAataTTAATCTATAAGCAATataACAAGAACAAATAACACGAAGGG
GCGAAATCTAACGCTAGATCAACTGCGTGGCTTTCACATCTTTGGTGGTCGAGTAATACA
TAGCACTAAACCGAAACAATTATTTTTCGTCGACTACCGTAATCCCCATTCC

Reference(s)