Aniseed - Construct

Construct 'CONS00001555' >
Construct 'CONS00000811' >
Clone 'AHC0AAA223YO12' >
Construct 'CONS00001661'

Construct

ID

CONS00001661

Name

p.Cirobu.REG.Kh2012.C1:823069-823390>LacZ

Min Promoter

bpFOG

Reporter

LacZ

Comment

The 322bp regulatory region located upstream of Nkxtun3 (Nkxtun3-Up5) was cloned upstream of the basal promoter of Fog and the reporter gene LacZ. It drives LacZ expression in dorsal and ventral neurogenic midlines (>20 and 40% of the embryos respectively).

This construct is testing Regulatory Region

REG00001566: Cirobu.REG.Kh2012.C1:823069-823390|Nkxtun3

Sequence

Show sequence

Length: 321

AAGATGGCGGGCGCTTCTCATCTGGCTTGTTTGGCAACAATTGATCGATAGCCAAATCGC
ATTATGAGTCATAACTAAAATTCGAATGCGGGTGGTGAATTCGAACAAAAACGTCGCGCT
AACTCGACAGCACTATTGCGTCATAAAAAGTCGTAAAGGCGCGCGCTTAAACGAGTAATT
ACGGAAAAATTATGACATCGTTTAAATTTCCTACAACTGTTTACGTCGTAAGCGTCTCAT
TCATTGTGGGCGTTTAACAAAGTAAAGACCGAAAGGTGACAATTATCCAGTAAAACCCAG
CCCAGTCAAAATCATCAGGGC

Reference(s)

Coulcher et al. (2020). Conservation of peripheral nervous system formation mechanisms in divergent ascidian embryos. Elife 9():

Show in situ data