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- Construct 'CONS00001645'
Construct
ID
CONS00001645
Name
p.Cirobu.REG.KH2012.L59:1807-2057_FoxMut>LacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
The 250bp cis-regulatory element of Zic-r.b (KhL59.1) in which the 4 Fox binding sites were mutated drives weak LacZ expression in aMM (B6.2 lineage) and normal expression in pMM (B6.4), compared to WT construct.
Show sequence
Length: 250
AACCACTCTGCAATGTcgcACTTGTgcgTGACTTCACGTAGAGAAAGTGCCATAGGTAAA
CCTTGCGTAGACAGGCACGAGCGAAAGCGTGCCAAAAGTACCGCGAGACGAGCGATCGAC
ACGATAATTACCTTCGCGATGTAccgaTCGGAGGTGGGGGGTCGAACCACGTGCGCATGG
GAAAGCGACGGGCGATCGGAAGTCAAAgcccTAAAGATAAAGAAGATAATTAAGTCATTG
TGTTACGAAA