Aniseed - Construct

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Construct 'CONS00001644'

Construct

ID

CONS00001644

Name

p.Cirobu.REG.KH2012.L59:1807-2057_GATAmut>LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

The 250bp cis-regulatory element of Zic-r.b (KhL59.1) in which the two GATA binding sites were mutated drives weak LacZ expression in aMM only(Muscle, mesenchyme and notochord, B6.2).

This construct is testing Regulatory Region

REG00001560: Cirobu.REG.KH2012.L59:1807-2057_GATAmut|Zic-r.b

Sequence

Show sequence

Length: 250

AACCACTCTGCAATGTGTAACTTGTTGTTGACTTCACGTAGAGAAAGTGCCATAGGTAAA
CCTTGCGTAGACAGGCACGAGCGAAAGCGTGCCAAAAGTACCGCGAGACGAGCGATCGAC
ACGATAATTACCTTCGCGATGTAAATTTCGGAGGTGGGGGGTCGAACCACGTGCGCATGG
GAAAGCGACGGGCGATCGGAAGTCAAATAAATAAAGcgAAAGAAGcgAATTAAGTCATTG
TGTTACGAAA

Reference(s)

Imai et al. (2016). Antagonism between β-catenin and Gata.a sequentially segregates the germ layers of ascidian embryos. Development 143(22):4167-4172

Show in situ data