Aniseed - Construct

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Construct 'CONS00001642'

Construct

ID

CONS00001642

Name

p.Cirobu.REG.KH2012.L59:1807-2086_GATAmut2>LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

The 280bp cis-regulatory element of Zic-r.b (KhL59.1) in which the two GATA binding sites were mutated (aGATAa replaced by tGATAg) drives very reduced LacZ expression in all the marginal cells.

This construct is testing Regulatory Region

REG00001558: Cirobu.REG.KH2012.L59:1807-2086_GATAmut2|Zic-r.b

Sequence

Show sequence

Length: 279

ACGTCCTGTTTTTTGGTCTTTTTTGCATAAACCACTCTGCAATGTGTAACTTGTTGTTGA
CTTCACGTAGAGAAAGTGCCATAGGTAAACCTTGCGTAGACAGGCACGAGCGAAAGCGTG
CCAAAAGTACCGCGAGACGAGCGATCGACACGATAATTACCTTCGCGATGTAAATTTCGG
AGGTGGGGGGTCGAACCACGTGCGCATGGGAAAGCGACGGGCGATCGGAAGTCAAATAAA
TAAtGATAgAGAtGATAgTTAAGTCATTGTGTTACGAAA

Reference(s)

Imai et al. (2016). Antagonism between β-catenin and Gata.a sequentially segregates the germ layers of ascidian embryos. Development 143(22):4167-4172

Show in situ data