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CONSTRUCT

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  1. Construct 'CONS00001639' > 
  2. Construct 'CONS00001641' > 
  3. Construct 'CONS00001643' > 
  4. Construct 'CONS00001642' > 
  5. Construct 'CONS00001638'

Construct

ID

CONS00001638

Name

p.Cirobu.REG.KH2012.L59:1807-2488>LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

The 681bp cis-regulatory element of Zic-r.b (KhL59.1) drives LacZ expression in NN (notochord and nerve chord, A6.2 and A6.4), aMM (Muscle, mesenchyme and notochord, B6.2) and pMM (Muscle and mesenchyme, B6.4) lineages at the 32-cell stage.

This construct is testing Regulatory Region

REG00001554: Cirobu.REG.KH2012.L59:1807-2488|Zic-r.b

Sequence

Show sequence

Length: 681

TAGTTTATGTCATTTCCGTTCACTAAATGATATTTAAATTATAATCCTCTATAATCTTCA
CACACGCTACAATGGCTCGAGACAACCCGAATTTTAAACAATGATGACGTAACAATTAAC
AAAGGGTCTGGAATGTTGAATTCGCAATGACTTGCTGTTATCTAGGTTGTTATAAGATTA
TTTTATAATTTTAAAAAGTTTAAGAACATGCATATAAAAAAAATCTGATAAACTATAATT
TTAACACAATCGGTATGCTAATATTAGTAGTTAAATCAAACATAAAAAAATAAGCCGGCA
AATACATGCAATATTTTTTATTAAACAAAATAATTAAAAATCGAATACGTAAAAATTATT
AACAATTATAATTTATTATATATTTTTTTTAAATATCGATGAACGTCCTGTTTTTTGGTC
TTTTTTGCATAAACCACTCTGCAATGTGTAACTTGTTGTTGACTTCACGTAGAGAAAGTG
CCATAGGTAAACCTTGCGTAGACAGGCACGAGCGAAAGCGTGCCAAAAGTACCGCGAGAC
GAGCGATCGACACGATAATTACCTTCGCGATGTAAATTTCGGAGGTGGGGGGTCGAACCA
CGTGCGCATGGGAAAGCGACGGGCGATCGGAAGTCAAATAAATAAAGATAAAGAAGATAA
TTAAGTCATTGTGTTACGAAA

Reference(s)

Imai et al. (2016). Antagonism between β-catenin and Gata.a sequentially segregates the germ layers of ascidian embryos. Development 143(22):4167-4172

Show in situ data