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CONSTRUCT

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  1. Clone 'AHC0AAA233YB02' > 
  2. EST 'AHC0AAA230YG15_RM1' > 
  3. EST 'AHC0AAA230YO05_RM1' > 
  4. Construct 'CONS00000596' > 
  5. Construct 'CONS00001634'

Construct

ID

CONS00001634

Name

p.Cirobu.REG.KhC11:1340971-1342802>TdTomato

Min Promoter

Endogenous Promoter

Reporter

mCherry

Comment

This plasmid contains a sequence of the Ciona Fucosyl Transferase control region that drives TdTomao expression in the b-line from late gastrula stage.

This construct is testing Regulatory Region

REG00001553: Cirobu.REG.KhC11:1340971-1342802|Fucosyltransferase-like

Sequence

Show sequence

Length: 1,887

GGCATCATAACGTACAACCTGATCTTGTTTTTCTACATTGctatTGCTTATATTGTAATT
TGGAAACATGCCACAACGTCGTTCTCGTTTAGACAAGGGTTTTGTTTATGTGGAAACAAT
GAAAACTGTGGCGAATCTTCACGGCGGAATAAACGAGAACAAGATAATCTATCTTTACAG
CGACGAATAATGGTTGTCATTGTGACGGACTTCCTGTCATGGGTTCCTTTATGTTGTCTA
TCTGTTTATTACGTTATAGCGACAAGTTTGATCGAACAAAGTGGATGCAATTATCAAGAA
TATACCGAAGGTTGGACGAAGATATTTTCAGCTTACACATTCGTTGCTATTCCAATAAAT
AGCTCCATCAATCCATTCATATATTCGACATCTTACTGGAGAGATGTCCGGTCACGTGTT
CGTCGATTTTCTTCCACCAAAAGAAGCCCAGCCAGTGAAATAGTTCATGCATTGGAAGGA
AATCAGCTTTAAAATCCTCACAAATGGGAATTAAACAAAATGCATGTCTTAACATAAAGT
TAAGATACAGACCCTAACATTGTTTTCACTAATGTTTCCGCATGGGCCATTTCAAAACTT
TGTTTCCCGAGTTAAATCAGAAGTATATTCACCATGGCCAAGTAGAACATTGGGCCATAG
CTTCAACAACTCTTTGTACATTATATAGCATTGTGGGTTTCGATAGATATCGTAATCACA
ACCCTTATTTCCTTATCGTTTTTTCAAATTAACAACGTTTTTTTTAGAGTCGTGACTCGA
TTATAAAAATCTGTAAATATTCTTGGTTCAATACCAAATGTGACGATAAAAGAGTATGGA
ATTATGTCCCATCTTTCCCCGCCCCTATTACTaaaATCTTGAttttAAATATAACCGTTA
aaatcaggtactgtttattatattgcgcttccgggcgagtgcggagtcactggctttagg
aaactgcgttcgctaatcaattaaccacaatgttttgttggtctcatttaccagtcgctt
tgcatgtaacacgtgatatgTAGACGCATAttaaAATATAaaTaccaattAtaacAGTAA
CATGTtCATAGTtGTcgTAATATtaTTgATTGTTATGcTGAccGTCATTAAAGAGaacta
TGTCGCCGTGTGTCATTCTGGCTTGTTTTAACCAAATCGAGGGTAACTTTGGATTTGGCT
TTTCTGCATGAACTTTTTTGTAACAATAAATAGTGTCTAAAAACGTATGACTGGTTTATA
TCAACTGAATACACCGGCAAAATGAAGATAAGGTAAATTTCGTAGCGGCCGAAATAATAT
ACATTTATTCCTGTACAGGTCCAACCTTTAAAGCTTAAAATATATGGTTTCGCTTTTGCA
AATCGAAATACAGCTATTTGGATTTGCATAAGAATTATAAACTACTACTACGTCACAATG
TCTCGCGGGCACGTCGCGATGATATGGGCGGCAGTCGCGGATCGCCAACGGCACTTTAAT
CGCGCCATGCAACGATTGTTTTGTAACGATCCTTCAATTTCTGGAACCATGTTTTCAAGC
TCTTAGTTCGCAATTCTTTGATTGTTGTTGGTATTGTTACGTAATAAACGGGTGCTAAGC
TTCGAAAAAGGTAGATGGAACAAGTAAGACAATAAATAGCTACGTGGTATTTCGTTCACT
CAAGCTCAGGTTGTATTACATGCGCTACACGAGCGATAGAAGGTTCATATTTTATACAGA
AACTAGAACATAGTAATAAATCGCATAACCAGCAAATAGGTGTGACGTCAGTATTTAGAA
AATTTAATATATTAATGAGTTGGGATTGATGATATTTCTTGTTTACCGATAGCGCTTTCT
TAGATAATAGTAAACTCTACCGCTGCA

Reference(s)

Hudson et al. (2016). Co-expression of Foxa.a, Foxd and Fgf9/16/20 defines a transient mesendoderm regulatory state in ascidian embryos. Elife 5():

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