Aniseed - Construct

Construct 'CONS00001618'

Construct

ID

CONS00001618

Name

p.Cirobu.REG.KH2012.C12:1333829-1333859_CloxMUT>LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

The conserved putative Clox binding site of the J-A enhancer (located -604/-573bp upstream of Rx TSS) was mutated. Cloned upstream of of the human β-globin basal promoter and LacZ reporter gene, this element is no longer able to drive reporter gene activity.

This construct is testing Regulatory Region

REG00001544: Cirobu.REG.KH2012.C12:1333829-1333859_CloxMUT|Rx

Sequence

Show sequence

Length: 31

ATCGCGTTTTCATTcTccCcAcAATACCCTT

Reference(s)

D'Aniello et al. (2011). Onecut is a direct neural-specific transcriptional activator of Rx in Ciona intestinalis. Dev. Biol. 355(2):358-71

Show in situ data