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CONSTRUCT

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  1. Construct 'CONS00001607'

Construct

ID

CONS00001607

Name

p.Cirobu.REG.KH2012.C12:1333114-1334401>LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

The C1.3 cis-regulatory element of Rx (KH.C12.152) was cloned upstream of LacZ reporter gene. This reporter construct is active in the central nervous system of Ciona robusta tailbuds and larvae. It also drives some ectopic activity in the mesenchyme.

This construct is testing Regulatory Region

REG00001533: Cirobu.REG.KH2012.C12:1333114-1334401|Rx

Sequence

Show sequence

Length: 1,287

ACAATGGAACCATGGAAAGATCTTTTGTCCTATATACTGAAGAATATACGATTTCGAGTC
ACGACGCTGCTACACCATTGTGGGCGTATATGTGTTTTTAAAAATAATTTATTTTCGGAA
ATTGCTTCAACGTATTGTTAGATTATGGGTTGTCTAAAGTTTTAGTCCCACATTAAAAAA
CAATTCAAAAGTAATTCGTAAGCAAGCACGATGTAATTAAGGCTAACCGCGTTTTTTACG
CCATGCGAGGATATACAAGTTACATTTATTCAATAACACTGCATCGTTTCTAATAACAAT
GATGGGTTACATTACAATAACATATATATACGTACTTATAGTCAAGACAAACTATAGTAG
CCGTGTTTTAGTCTATTACCCTACTGCACGAAACATCTTTTGAATATCATTTTTAGCATC
CAGACAGTAACTTGGTCCATCACCAGTGTAAAAAAGGTTGCTATCCACTAAGTTACTGTC
TGAAACATCTGCTGATCATGAGTTTATTATCGACGTATTGTTAAACTAAAACATAGTCAA
ACGCAAAGTCTTAATCGTAACACATTTATTTTAGAATATTATAAAAAACGGTACCATACT
TTACCGATGTTTTTTAAAATTTGTTTGTTTAATTTTGATACTTCCTTCAGCCCATTTGTT
TTTGTGTGTGCTTATGCGGTACAATCTAAATTATAATTATTCCCTTAAGAATCGATCGCG
TTTTCATTTTATCAATAATACCCTTAAGGGTCAGCAAGTATGAAACGCAATTAGTGCAAA
TTGCCCTAATCCTTATTAAAATGTCGAAGTATTATAACGAGTAATTTAACATAATTAATA
GAAGAGACATAGCAGTGCATTTTCAAGTAGAATCAATACTAGCATTACCATCTGGCGATA
CACGGCCATATATACTCGGCCCGGCTGCTATAATTTTTCAATAATTCCTGCTCACACGTC
GTAATTCGTACTAAACAGATTGCTCTTTGTGATTTTCAATCACTTCCAAATCTTTGCTTA
CTCTTTAAACTAGGAGCGAAGAAAGGAAAAAGGCAATTTGTTCCAGAACCTGGTTAAAAC
ACAGTGCTTATGACGACATAGCTGTAATACTGAAGTGATTGTTCGGTAAGAAAGAAGTGA
ACTCAAAGCGGACATTTTACAGTAAGGCTTAGAAAGTCAAGTACAGTATATTTAATAATG
CATTCTAAAGCATATTTCATGGACACTTCATTCATTTTATTATTATAAAAAAATTAATTA
GATTTTAATTCATATTTCGTTATTTAG