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CONSTRUCT

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  1. Construct 'CONS00001584'

Construct

ID

CONS00001584

Name

p.Cirobu.REG.KhC1.9789507-9790838>unc76::CFP

Min Promoter

bpFOG

Reporter

CFP

Comment

The -3.5/-2.1kb region located upstream of Dmbx TSS, cloned upstream of the basal promoter of FOG (KH.C1.1212) drives CFP expression in descending decussating neurons (ddN) at larval stage in Ciona robusta. The Unc-76 tag is used to label axons uniformly.

This construct is testing Regulatory Region

REG00001515: Cirobu.REG.KhC1.9789507-9790838|Dmbx

Sequence

Show sequence

Length: 1,332

CACTCATCTGCCTACATTAGCTTAGTATTTTAGTTGAATAAATAAGTTCCAATATTTTCA
CAACCCGTTCTTAACTATAGAACAAGAATCGCTACGGCTCCGATGCTCGATTTCGAGTAC
CCGGTCGCCCTAGCAACCCACTTCACAATAGTAACAATGAGACCCGACCCTCCCATTGTG
AGGTAATGTTTCTTTCATTGTTACGTGATATCGAAACTCCGCCAATCGCAAGGGAAAGAA
AATCGACAGGGCACAAAATGAAATTTTTCGAAGAGTTAAAAACGATAAAAAAACAAACTA
AAAATACTAAAAGTTGTTGATTGTTATAAAAACGCGACAGAAGCGTTTATTGTTTTATCA
CAATGACGATTAACCGGACCATACTATGCCATTGAAACCGCCTAATTGAAATTTCGTGGT
CGGATTCGCTATTTTCGCACCCTGCGTTCCTCAATTTGATTGTATTCTACATCGTTAATA
TTTAATGAAATATTGACTTGTTAATCGACACTTCGCGAAAGAACAATTAAATCGTTATGA
CGTCATATGGGTAGACGATTGTTTTAATCGCGATGTTTCGACACCACCTGTTAAAATGTT
TAACGAAAGAAAATACTAAATCAAACTTTAGTACTTTAAACGGCAATTGTTTCAACTTCG
TATTCACTTACGTCTTAGGAGTTGTCTAATTTGTCAGCCATACAAAGGGGTAAAGCCAAA
AAATAATCACTCACATAGTTACATACATATGGTAACTCGTAAGCTGGCACGAGGTGTATG
AAACAGAACACTCATGTTATATACGACGTTGGTCATAACGACTGTCGTTTTCCGGCCACG
CGAGGATAAATAAGTTGCATTTACTCGGCCCATTTAGTATAAAGAAGTAAAAAACGTTTT
TATAATAAGCAAAAACTAATCAATTAAAAGAAGTTTTTAAACAGATCAGGTTGATCGTAT
TTAACCATGGATAAGAATACAATTCAGCCAATTAAAGTTTCGAATTTAAAGTTGATTTAT
TTTAATTTATTCGAATTAACAAGAAGTCGCCGATAAATCGAAGACACATTTGTGACGTCA
CGCCTTAACAATCCGCTATTGATGACGTAATAAAGGTGAATCATCTGCCATTATGAGCTC
ACAATTAACGCGTCTGATCTGCGACAAACGAAGCGCGAAATTGCTCGACGCGCTGGGAAT
AATTAACCTCGCTTTAACGCCATCTAGCGAGCACGAATTCCTGTATATTTAGGAGCGTAA
ATAACCAGAATACTCATTCGTGGTTAACAATCAGCATTGTTACGTCAATAAGGATTGTAA
CGTCATAGTACT