Aniseed - Construct

Construct

ID

CONS00001317

Name

p.(Cirobu.REG.KH2012.S654:26922-27123_μE)x4>GFP

Min Promoter

pBra

Reporter

GFP

Comment

Four repeats of the critical upstream sequence of Tbx6.b with mutations were ligated to the Brachyury basal promoter upstream of GFP.

This construct is testing Regulatory Region

REG00001297: Cirobu.REG.KH2012.S654:26922-27123_μE|Tbx6.b

Sequence

Show sequence

Length: 812

TTATCAAACCAACGCGCCACATGCAAGACGGTATGCGTACACTGAGTTTTGGAGTGTTCT
GCATGCGCTAGACACGAATCAGCAGGAGAGTTCGGAGTCTTATCAGGAAGCAGTTGTCCT
TGTTAATGACTCGTTAAGATCAAAGTGTGTAGGCTGATGAATCTCGCTATAAAATGGGTT
TAGTTTCACAGTACCTCATTCCGTTATCAAACCAACGCGCCACATGCAAGACGGTATGCG
TACACTGAGTTTTGGAGTGTTCTGCATGCGCTAGACACGAATCAGCAGGAGAGTTCGGAG
TCTTATCAGGAAGCAGTTGTCCTTGTTAATGACTCGTTAAGATCAAAGTGTGTAGGCTGA
TGAATCTCGCTATAAAATGGGTTTAGTTTCACAGTACCTCATTCCGTTATCAAACCAACG
CGCCACATGCAAGACGGTATGCGTACACTGAGTTTTGGAGTGTTCTGCATGCGCTAGACA
CGAATCAGCAGGAGAGTTCGGAGTCTTATCAGGAAGCAGTTGTCCTTGTTAATGACTCGT
TAAGATCAAAGTGTGTAGGCTGATGAATCTCGCTATAAAATGGGTTTAGTTTCACAGTAC
CTCATTCCGTTATCAAACCAACGCGCCACATGCAAGACGGTATGCGTACACTGAGTTTTG
GAGTGTTCTGCATGCGCTAGACACGAATCAGCAGGAGAGTTCGGAGTCTTATCAGGAAGC
AGTTGTCCTTGTTAATGACTCGTTAAGATCAAAGTGTGTAGGCTGATGAATCTCGCTATA
AAATGGGTTTAGTTTCACAGTACCTCATTCCG

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045

Show in situ data