Aniseed - Construct

Region 'REG00001123' >
Construct 'CONS00001316'

Construct

ID

CONS00001316

Name

p.(Cirobu.REG.KH2012.S654:26922-27123_μD)x4>GFP

Min Promoter

pBra

Reporter

GFP

Comment

Four repeats of the critical upstream sequence of Tbx6.b with mutations were ligated to the Brachyury basal promoter upstream of GFP.

This construct is testing Regulatory Region

REG00001296: Cirobu.REG.KH2012.S654:26922-27123_μD|Tbx6.b

Sequence

Show sequence

Length: 812

TTATCAAACCAACGCGCCACATGCAAGACGGTATGCGTACACTGAGTTTTGGAGTGTTCT
GCATGCGCTAGACACGAATCAGCAGGAGAGTTCGGAGTCTTGTGTAGGCTGATGAATCCT
TGTTAATGACTCGTTAAGATCAAAGTGACATCGAAAACGAGTCTCGCTATAAAATGGGTT
TAGTTTCACAGTACCTCATTCCGTTATCAAACCAACGCGCCACATGCAAGACGGTATGCG
TACACTGAGTTTTGGAGTGTTCTGCATGCGCTAGACACGAATCAGCAGGAGAGTTCGGAG
TCTTGTGTAGGCTGATGAATCCTTGTTAATGACTCGTTAAGATCAAAGTGACATCGAAAA
CGAGTCTCGCTATAAAATGGGTTTAGTTTCACAGTACCTCATTCCGTTATCAAACCAACG
CGCCACATGCAAGACGGTATGCGTACACTGAGTTTTGGAGTGTTCTGCATGCGCTAGACA
CGAATCAGCAGGAGAGTTCGGAGTCTTGTGTAGGCTGATGAATCCTTGTTAATGACTCGT
TAAGATCAAAGTGACATCGAAAACGAGTCTCGCTATAAAATGGGTTTAGTTTCACAGTAC
CTCATTCCGTTATCAAACCAACGCGCCACATGCAAGACGGTATGCGTACACTGAGTTTTG
GAGTGTTCTGCATGCGCTAGACACGAATCAGCAGGAGAGTTCGGAGTCTTGTGTAGGCTG
ATGAATCCTTGTTAATGACTCGTTAAGATCAAAGTGACATCGAAAACGAGTCTCGCTATA
AAATGGGTTTAGTTTCACAGTACCTCATTCCG

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045

Show in situ data