Aniseed - Construct

Construct 'CONS00001312'

Construct

ID

CONS00001312

Name

p.(Cirobu.REG.KH2012.S654:26922-27123)x4>GFP

Min Promoter

pBra

Reporter

GFP

Comment

This constructs contains 4 times the 200pb enhancer of Tbx6 (KH.S654.3) concatenated. It is cloned upstream of pBra and GFP.

This construct is testing Regulatory Region

REG00001111: Cirobu.REG.KH2012.S654:26922-27123|Tbx6.b

Sequence

Show sequence

Length: 812

TTATCAAACCAACGCGCCACATGCAAGACGGTATGCGTACACTGAGTTTTGGAGTGTTCT
GCATGCGCTAGACACGAATCAGCAGGAGAGTTCGGAGTCTTATCAGGAAGCAGTTGTCCT
TGTTAATGACTCGTTAAGATCAAAGTGACATCGAAAACGAGTCTCGCTATAAAATGGGTT
TAGTTTCACAGTACCTCATTCCGTTATCAAACCAACGCGCCACATGCAAGACGGTATGCG
TACACTGAGTTTTGGAGTGTTCTGCATGCGCTAGACACGAATCAGCAGGAGAGTTCGGAG
TCTTATCAGGAAGCAGTTGTCCTTGTTAATGACTCGTTAAGATCAAAGTGACATCGAAAA
CGAGTCTCGCTATAAAATGGGTTTAGTTTCACAGTACCTCATTCCGTTATCAAACCAACG
CGCCACATGCAAGACGGTATGCGTACACTGAGTTTTGGAGTGTTCTGCATGCGCTAGACA
CGAATCAGCAGGAGAGTTCGGAGTCTTATCAGGAAGCAGTTGTCCTTGTTAATGACTCGT
TAAGATCAAAGTGACATCGAAAACGAGTCTCGCTATAAAATGGGTTTAGTTTCACAGTAC
CTCATTCCGTTATCAAACCAACGCGCCACATGCAAGACGGTATGCGTACACTGAGTTTTG
GAGTGTTCTGCATGCGCTAGACACGAATCAGCAGGAGAGTTCGGAGTCTTATCAGGAAGC
AGTTGTCCTTGTTAATGACTCGTTAAGATCAAAGTGACATCGAAAACGAGTCTCGCTATA
AAATGGGTTTAGTTTCACAGTACCTCATTCCG

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045

Show in situ data