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CONSTRUCT

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  1. Construct 'CONS00000532' > 
  2. Construct 'CONS00000208' > 
  3. Construct 'CONS00001117' > 
  4. Construct 'CONS00001311'

Construct

ID

CONS00001311

Name

p.Cirobu.REG.KH2012.L13:47071-47600_Bra2mut>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives no LacZ expression in the notochord.

This construct is testing Regulatory Region

REG00001292: Cirobu.REG.KH2012.L13:47071-47600_Bra2mut|KH.L13.2

Sequence

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Length: 530

ACCTGGTATATGCTGGAATAATGAACATATTTAACTCATATTCATTTGAAATAACGGCCT
ATTGAATTTAAAGCGATAATTGCCCTTTAAGAGCTCACGTCCATAACGCTTATTGCCTCT
CCATCTTTGGAACTGACTCTGTACATAAAGTGGCTTCAACTAAAACCTATTTAAAATCTA
TCACTTTACTGAGAAGAGTCAAAGACGAACATTGGCCGGGAAGTGGGTGACCGTTGAATC
CGGCTTATCCACGCTGGCTGACTTACCAGATCGCTTATTAACTTTTTAACTCCATTACGT
CCGTTATCAAGCTGACAACTGGCGCGAATGGAATCGTAACTCTATATATATTATAACTCA
ATCTACCGAGACGACGGGTTATCGAAGAAAAACATTCTTGTCTGGCACTCTCTCACCTAA
GTGCCTTATAAGGAGTTAGTCTCTCGACTTAGTAAAAAGTAACTAACGTCGCTGCGAAGA
GTGATACTGGAGTAACAATGACTCATTAAGCCGCGATAACCAAATGCTAC

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data