Aniseed - Construct

Construct 'CONS00001285'

Construct

ID

CONS00001285

Name

p.Moocci.REG.ELv1_2.S145165:64532-65350>GFP

Min Promoter

Undefined Minimal Promoter

Reporter

GFP

Comment

1.2kb Mesp regulatory sequence drives GFP expression.

This construct is testing Regulatory Region

Sequence

Show sequence

Length: 1,176

5’GTGTTTAGCGATCACGGTTCAAGTTTAGGCTACATCGCTTTTTCCATCGGTTTGCC
TAAACTTGCATGCTGTATACAGTATTAAGAAGGTCTTGTTTAACAATGTTCCTTAAATGT
TATTGACATACAAAGCAAACTTTGTGTTAAAAGCCTACTTTAATGTGAAGACCAACATAA
AAAAATTATCGGATAGTATAACTTACTCAAAAAAGTTAAGTCTTTGTCCTCTTTGCAAAG
CTGCAGTAACTTTATCGAATCCCCCAAGATACACGGATGTTCTGATGATAACTAGAAGCA
TCCCCACTATCATTACTCCAGCTTGTACAACGTCTGTCCATATCACACCTTTTAAACCAC
CCTGGAAATTAAATCATTATTATTATTAAAGATAAAAACCATTTATAAGCCTATTTACAA
TACAGTTCCAAATTTAATCTGCCAATTCGATTGAACGTTTTTCAATGAAGTAAAATATCT
CTCACAATCTTGTATTGAGCCTTTAAAATAGCCTTTTAAATGAAAATCTATATCTGACAA
AATAGGCTTTGACGTGATTTGAACGCCGTACCTCATGCTCGTATAAATACCTAATTTGTT
GCGGAATTTCTACTTCAATATAAAAAGTATGCAAATACATACATAATAGTTATTGAAAAG
AATTTAAATCATCAAATTTTATGTCTGTTGTAGCCTAAATAGTGTTTTATTTAAGTAAAT
CTACACTGCGCTTCAGTTCAAACCAACCGTGCCTTGAACTACGGGCAGTGCGGTTGCTAT
AGACGTTATGCTTACGTAAACTAGTGCAAAGAATTTGTTGGAAAGTGTTAATTGATATCC
GACCACCGACTGTGTTATCTTATCGTTTTGATAGATAAGAGCTGATAACACTGATAATAG
ACTAATTAACTATAGCAGTTAATTTTTATTAAAATTTTCACTTTTCGGACATGAAAATTT
GTCTAAAACCCAAACTTCCAAACTTCATCTATATAAGGAAAGAATTTTATTGAAATGGCA
TACTCAATTCATCAGTATATATCATCTATATCTATATACGGTGGGTAATACGTCGTAATT
GATATCGGACAAAGTAAAAACTGCATATTGAAGCGTTAGAAGAAAGTTGTTCAATTCAAC
ATTTTAACAAAATGGACCAAAGTGTACTGGGA3’

Reference(s)

Stolfi et al. (2014). Divergent mechanisms regulate conserved cardiopharyngeal development and gene expression in distantly related ascidians. Elife 3():e03728

Show in situ data