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CONSTRUCT

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  1. Region 'REG00001017' > 
  2. Molecular Tool 'Ci-FoxD/Ci-lefty' > 
  3. Molecular Tool 'pGsx>OC::Vp16 (Vassalli 2021)' > 
  4. Region 'REG00000202' > 
  5. Construct 'CONS00000993'

Construct

ID

CONS00000993

Name

pCirobu.REG.KH2013:KH.C4.1881731-1882173>NLSLacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

The regulatory region contains 399-bp 5' flanking region, 5' UTR and 38-bp coding region. The DNA fragment was fused in frame with the translated region of lacZ.

This construct is testing Regulatory Region

REG00001024: Cirobu.REG.KH2013:KH.C4.1881731-1882173

Sequence

Show sequence

Length: 443

CTAACACTATACATCTTAACCCATAGTAGCATAGCATGGATTTTTATTGTTTCTTGTTCC
CTCCGTGCCGGGAATTGTTATCAGTCAAAACAAACGCAACGAATCGGCCAAAATCTGCTT
GTTCACATGCAAAACTGTCAACACAAAAGTGACAATTACCGAGTTTACACGCATGTAAAA
TTATTGTAAGAAACCCGATAAGGAGACCTACTTTATCGGCCCCTTATCGTGGAGATTTGC
AACACGCTCTAGCGTTTCAGCCGAAAGCTGTAAGCTTGTATAGTGTTATTGCTAATCACT
GTTAGTTGTTGTTGTTGCTGAAAAATGAAGAAGTTATCAGCAAAAAAAGTTGAGTTAGTG
TTGGCAGTTTAATAATACTGAGCCGTTGAAAGTGTTGGGTTAAAGATGCATCGAATACTG
CTAGCTGGTTTGTGTTTATTCGT

Reference(s)

Horikawa et al. (2013). Transcriptional regulation in the early ectodermal lineage of ascidian embryos. Dev. Growth Differ. 55(9):776-85

Show in situ data