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CONSTRUCT

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  1. Construct 'CONS00001218'

Construct

ID

CONS00001218

Name

p.Cirobu.REG.KH2012.C6:403548-403985>LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

This cis-regulatory sequence of Onecut/HNF6 drives LacZ activity in the sensory vesivle, the visceral ganglion, ectopic mesenchyme, and weak activity in the tail of Ciona intestinalis tailbuds and larvae.
A human β-globin basal promoter is localized between the regulatory sequence and the LacZ reporter gene. (It corresponds to B construct in Pezzotti et al. 2014)

This construct is testing Regulatory Region

REG00001216: Cirobu.REG.KH2012.C6:403548-403985|Onecut_HNF6

Sequence

Show sequence

Length: 438

ACAACGAACTCCGAAGAATTCAACGTCGCCATTTATTTACGATCATGCATGTCGCTGCGT
ATACTTATATGCTGGTTATGCACGTACCTTTTATAAAGTATATATAAAATTACTTTTATT
TAGTTATATAGTCGAAAAAATATTATCAAAAATATTCTTTTTTTCGAAAATATTTTAATT
TCTCTCTTGCGCTATGCGAACTTGAATCTTAGAAACTGTAATTAGGCCTCCAAACTGACT
AAACTGTTTTATAATTGAATATTTTTCTTTCCAAGGCTTGAACTTGTCTTAATTGTAGAT
TGTTTGAATTTAAACAAATTTTCGATCAGTCCTTTACGGTTGAGCTCCAAGCTCGAATGG
AAGACTGGTGCCGTACTCGTATGGGAGTAAGCAATGGGCCATCTGCTGCGTTTTTCTGTT
CGGCTTATATACGAGTGG