- Construct 'CONS00001218'
Construct
ID
CONS00001218
Name
p.Cirobu.REG.KH2012.C6:403548-403985>LacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
This cis-regulatory sequence of Onecut/HNF6 drives LacZ activity in the sensory vesivle, the visceral ganglion, ectopic mesenchyme, and weak activity in the tail of Ciona intestinalis tailbuds and larvae.
A human β-globin basal promoter is localized between the regulatory sequence and the LacZ reporter gene. (It corresponds to B construct in Pezzotti et al. 2014)
ACAACGAACTCCGAAGAATTCAACGTCGCCATTTATTTACGATCATGCATGTCGCTGCGT
ATACTTATATGCTGGTTATGCACGTACCTTTTATAAAGTATATATAAAATTACTTTTATT
TAGTTATATAGTCGAAAAAATATTATCAAAAATATTCTTTTTTTCGAAAATATTTTAATT
TCTCTCTTGCGCTATGCGAACTTGAATCTTAGAAACTGTAATTAGGCCTCCAAACTGACT
AAACTGTTTTATAATTGAATATTTTTCTTTCCAAGGCTTGAACTTGTCTTAATTGTAGAT
TGTTTGAATTTAAACAAATTTTCGATCAGTCCTTTACGGTTGAGCTCCAAGCTCGAATGG
AAGACTGGTGCCGTACTCGTATGGGAGTAAGCAATGGGCCATCTGCTGCGTTTTTCTGTT
CGGCTTATATACGAGTGG