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CONSTRUCT

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  1. Construct 'CONS00000249' > 
  2. Construct 'CONS00000556' > 
  3. Construct 'CONS00001096' > 
  4. Construct 'CONS00000696' > 
  5. Construct 'CONS00001231'

Construct

ID

CONS00001231

Name

p.Cirobu.REG.KH2012.C6:403782-404043(µ_Ngn)>LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

This mutated cis-regulatory sequence of Onecut/HNF6 drives LacZ activity in the sensory vesicle, the tail and ectopic mesenchyme in Ciona intestinalis tailbuds and larvae.
A human β-globin basal promoter is localized between the regulatory sequence and the LacZ reporter gene. (It corresponds to FG(NgnMut) construct in Pezzotti et al. 2014).

This construct is testing Regulatory Region

REG00001229: Cirobu.REG.KH2012.C6:403782-404043(µ_Ngn)|Onecut_HNF6

Sequence

Show sequence

Length: 262

CTGACTAAACTGTTTTATAATTGAATATTTTTCTTTCCAAGGCTTGAACTTGTCTTAATT
GTAGATTGTTTGAATTTAAACAAATTTTCGATCAGTCCTTTACGGTTGAGCTCCAAGCTC
GAATGGAAGACTGGTGCCGTACTCGTATGGGAGTAAGCAATGGGCGGTGGGGTGCGTTTT
TCTGTTCGGCTTATATACGAGTGGTTCGCGTCGGCGCGCGGTTTGAGCAAAAACAATAGG
ACCGCTGTCGAAAACAAGGCGA

Reference(s)

Pezzotti et al. (2014). Auto and cross regulatory elements control Onecut expression in the ascidian nervous system. Dev. Biol. 390(2):273-87

Show in situ data