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CONSTRUCT

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  1. Construct 'CONS00000056' > 
  2. Construct 'CONS00001653' > 
  3. Construct 'CONS00000652' > 
  4. Construct 'CONS00000672' > 
  5. Construct 'CONS00001134'

Construct

ID

CONS00001134

Name

pSP.Cirobu.REG.KH2012.C5:4201966-4204077(µGATA_AP2-1 deletion):prCirobu.bpForkhead>Kaede

Min Promoter

pfkh

Reporter

pSP-Kaede

Comment

This reporter construct contains the 2kpb region located upstream the start codon of cdc25 (KHC5.12), where both GATA binding sites of the CRM1 were mutated (as defined by Ogura and Sasakura 2016)and the first AP-2 binding site of CRM2 (ie. the closest to the +1 of transcription) was deleted, cloned upstream the basal promoter of Forkhead and the reporter gene pSP Kaede.

This construct is testing Regulatory Region

REG00001160: Cirobu.REG.KH2012.C5:4201966-4204077/cdc25_µGATA_AP2-1deletion

Sequence

Show sequence

Length: 2,103

TGCCTGTATAACAATGGCCCACCTGTGCCGTCACAGAAGAAAACAATGGACTTTTGAAGG
TGAAAGGAGAGGCAATTCGTTGAACGGTCCTATTAGAACGAATCCAGCTCTTATTTATGA
CGCTAGAATAGTGCGTGCCTCGGGGCCCGAACCGCGACGCTTCGGCCTTCGCTACTTATC
AGTGGACGACCAAAACACACTGAACCTGTAGTAAAGCGAGAAAAAGGCGACTGCACCAGC
ACTCTTAAAATAGCAGCTGCGGTTCGATGCAGGGCACCATTCATTCAACTGCACATGCAG
CGCACAACCAGCTGAGTTCAATTTGTTAGTAGATCGATTCGGTATACATGCATTATTAGC
AATATTCCATGTAATTGGAGAATATGCAACAGTGCAGGATAGTGACCGATTCGTTAATTA
AAACTAATCTAGTATTCACTCATGTTCTGCCTGCATAGTTAAAGGAAGACTAGTTGTGTG
TTTTTCGTGCAACGTTAGTAGGACAGTAGTAGTAAGCAAAAATACGTCGTCAATATATAT
GTGGTGCTTCTCGTAAATATCTCCGCGGAGCAAACTTTTCTATAAAAATTAGAACCGACT
CGCTGCCTAATCCGTAACTAGTTCCCAACTTCGGATATATTAAGCAAGAATCCCACCCGT
TAATCCACTTGCGTGCATCCATAGTGCCCGCATAGCGTACAAACAGCCAACCGAGACATC
CTGACTGCATCCAGCCGGGCCAAGGTGCGCTAGTATTGTTGGTCGTAGGATTTACTTGCC
AGCAGCAAACTCGCTACCGAGCGAGAGCCCAGCGGTATGTTTCCTTTTCGACTCACCGCC
AAAGGAAAAAGGTTAATCTCTCGCTTGGTAATCGCGTCACAGGTAAAGGTAGTCACTCGC
ACGCTGTGTCCCAGCTTGGCCGGATTGTAAGATGCTTCGTAAACCAGTTTACATGAGCTG
CCTTCAGAATATCAATTAGCGTCCAAACCGGACAATGCAAGGGAATTAGCTCATAGCTCT
TGGGCCTTTTTTCTCAACTTCCTAATCGTAGACATCTTGCCTGGGATTTCAGTTTGAGCG
CGTATTAAGCAGCGAGACAGACTTCGGCTCTTATATATTACTAGACAGAAGCATAAGAGT
CAAACAGTATTCACCTATTCAGCTACCTCTAGGTAGTTACTGGTCTACGTAAAACTTTAT
CATTACTGTACAACAATGTAGTACCGTTCCAGTATACCCAGAGCAGCGCATTGCAGACCA
CATACTACGCATTGTTATTGTTTACTTTGTGCCGATAAGCCGTATCCAATGTATATAGGA
CAAGTGGCCAGTATCGACAGGCGGATGTATTCACATCTAAAGCACATAACTGTAGCGTGA
GGCTACCACGTTATATAGCGACTATAGCGTATTGGTAACTGAAAGAAATTGCGTATGTAT
AGTAGTTTGTAGTGCGTTAAAACTAAAGTGGAATGCTCCTCGAGCACTGAGAGAAAGAGA
CTATGCTATTACATGGTTGTTTAACTGACGATACTAGACGGTTCATTGCACAATAACTAG
AATCTAAGAGTCGTATACTAGTTAGCTGGCTATAGTATTATTGGGGTTTTGGATATGTCA
TTTTATTGTGTTGATTAAGAGAGTACCATGCAGCATACATACATACGTTGTATTGTGTAT
TATATTGAGTGAGTAGTATTAGAATGTCGGGTATATACTCAAAAGGCCTCCTATACCTCG
AGCGTAACATATGGTGTGGCCATTGGAGCTTATTGCAACAGTTGTTCTGCGACGCAAGCG
TTATAAAGTTGGATTATGACTTAGTAAAGACCGCAATAACATAGTGGTCGTATTGCCGAT
GGAATTGGGTCATATCGGCGATGTAACTTATGGGCGTTTGTCATAAGCCGATATCTCGTG
GATTAGCAGAGTATCTTCGCCGATCGAGTAAGCAAACTGTTTTCTTTTGCAGAATTTCAG
GTTATTTGACATCCGAAGTAGCTAATTGCGTTTTAGACATACGGTATCTTGAGAGCTTAC
TCTGTTGTAAGCTAGCGGGTGTTATACACTTGTGGTCAATCTTCAGCACCTGTCTTTAAT
ATG

Reference(s)

Ogura et al. (2016). Developmental Control of Cell-Cycle Compensation Provides a Switch for Patterned Mitosis at the Onset of Chordate Neurulation. Dev. Cell 37(2):148-61

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