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CONSTRUCT

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  1. Construct 'CONS00001273'

Construct

ID

CONS00001273

Name

p.Ci-Hndx -2938/-56>H2B:GFP

Min Promoter

pfkh

Reporter

GFP

Comment

This construct contains the first 3kb of the 5 flanking Ci-Hndx promoter sequence (it starts in the previous gene). It was cloned into the pCES reporter gene containing the minimal Ci-Fkh promoter region and lacZ. GFP expression is observed in the nuclei of endoderm, trunk lateral cells, and trunk ventral cells.

This construct is testing Regulatory Region

REG00000158: Ci-Hndx -2938/-56

Sequence

Show sequence

Length: 2,882

CGTTCTTGAAACTGGAGACATCCTGGAAAAGAACGCCGAAGTTGCCAAGGGTTTGACCAC
GTGACCTTGAACCAAGGTTACGTCATGTTCGAAAAATCGAAATTTCCCGAAGGAGGGACA
GCGGGGAAGTCCAAAGAAAGCAAACGATGAAGATTCGCTTTATCGTGACGTCACGGTCGC
ATTTATGACGTCACGATCGCGACCCTTCGAACTTTTTTGTTTAAGAACATCGTACTATAT
TCGATACAAAAGTACCATTGTGACGTCACACCATTACCGGCACTTTTATGTTCTCGTGAC
GTCACAATATTGTTCCGTGTGAAACGTTCAAATATTTTTTAAGACAAATTTCACTCAATT
TTGAGATTATTGGATCGATGTCGATACTCTGGCAATGCAGCCGCTGCTTCATATTAAATT
TGGCCCCATTTTTTAATCATATCTTGGCTGCTTCATACGCATTGTGTGAGCAACGAAAAA
TACTTCTGCGGCTTAACGAGTGCAGCGTCCGATTTTTCGCAGTTACACCGTGTGTAAGTT
TTTATTGTACGTCCATCAGCACTGTACACACTTTCACCGGTACCCCTTCAATTATTTATA
TTTACCGACGTAAATTTGCTGTGATTAACATGCGGTACCGTTGGCAATATATATTAGTTC
AATTTTCGAACTAATTTAAAAGTGTCTTGAGAGAATTCAGAAGAAGATAAAATATACAAT
ACAAGGTAGCGGAGCTATAATAAATAGACACGCAAAGTATAAAATCATAAAGAAAAACTT
ATTGTATACAGAGCAGAAATCATGTGTGTGTCACAGACTTTTTTTGCCGCCATGGACGTT
TCTAAAACCTCTTAGGTCAAAGTAATTTTTCTTACGTACATTTTTTGTCTGTCGTGGATA
TTTTAAGTCCGTCAAAGACACTCTATTTTCGTCACTTTAAATTTGTTACAGACAAATTAT
TATTATCCTTCTTAAAGTAGTAACCGGTTCGCTTATCGATATAATTTCGAATCTTTAGAG
AACAGTCCTTAAGATTCGTATAAACTCATCGCATCGTTTGTTTTGATAACGAGTGGCATA
GGCCTCATTACGTCATAATCAATTAGAACGACTTCCTTATTACGTAGGATCTGTGAACAA
TCAAAGGTTTGACTTTACACGGTTACAGTTTGACCTTATGGAATTCTGAGACACGAATAC
ACATAAAACAAACATACACTACAACTTGAACACCACAACCAATATTTAAAGTAAAAATTA
TTGGGAAAAACTTGTTGCAAAAAAAATAACTCGTTTCGTATTAAATATTTTTTAAACCAC
ACTTTACTGCATTTGTGTAATATTTAATTTAAAAAGTTTCCGTAGACATTTAAGTCTGCG
AAATTTTATTACGATTATTTTACATTTATGTTTATACAGCCAAGTTTTTGGCCAAGATAG
TTCAGTGTGTTAAAATCGACGCCGGTTTTGTATTATATTTAGCCCAGCTTTTACACCCGC
CACATAATAGTAAACCTTGCACCAACCTTCTATCCGTCCCATTCACCTGCCTTCATACCA
GCTATCATGGCGGGTTAAACGGCTCGCATTTAACCGCTGCCTCGTAAAATGTGACCTCGT
CAGTACTTCCGCATTAAAAGGGGCGAAAAGTTACCACCTCGTTAAGTAGGAACTGAAACT
AATATAACGTTTTGAATCAGACCCAGTGGTCTACTATTTAATGTCTAAATTGTCAGCCAT
ACATAACCAAAATTTTAAGAATTTCCACAAAGGTATATCAAGGTAACTCGTAAGCGGGCA
CATAGTGCATGAAACAGAAAACCCGTGTTTTAACCACTCTCATTGCCGCGCCAAGGCAGA
ATAAAGAGCTACAATTTATTACATTCATTTAAGTGTATACGATGAATAAACAACAGTTAA
AGTTACGAGAATTAACCTAAACTTTTAACGTCATACCAAACTGGAAAAAACGACCCTGGT
ATACGGCAACCCGTTAAAAACGTTTGACCTGATTTTTTTAATAGTAACAGTTATACGATT
AGGTTTAGGGCCGAAACAACTGTCTAAAAGGTTTGTTAGGTTTAAATTATATATTGCTAT
ATTCCAGGCGCTTGGAAACGGCATTCAAAGCTTTTAAGGGGTTTTATAAATCACTTAACA
GATATACTTATTTACGTATGCGAAAAAAATTGAAACCCTAAGACTTTACTAATAAACTTA
ATAGTTAGAAGCGCGAATTTATCCTAAAAAGAAAGCTGAAACTATGAAGGTTTTTTTCAT
ATTGATACATGATAGTCTTTACTGCATTTGGGATCGCCGTTAGGACGATAATCCTCCACA
GTTATCTAAACAAGTGTGTCTCCCCTGACGCACAATGGTTGCAACATATGTCAAAATTGA
GCATTACAGTAAAAGTTGTTTCAGTCATTTGTGTTATCTGCAGAACAGACTGCTCGGTGG
GGAATTAATATTAGCTTCTCACCGTCCTACTATGGGATTGCTTCTCTCGTCTCGAGATAC
TCTGTTTGCGGTGCACCTCGCACAAAAGAACGATTAATATAATGTAAATAGTAAGTAATT
CCAAACGCAAACCGAACAAGCGTCGCGTTAATTCTCGAAACGATACTGTGACGTCACAGA
AGCGTTTTCCTAATTAGTCGCATCGCCTTTTTTCAACGTCGGACGATCGCGTGAAACGGT
GTATATAAAACGAAGGCGCGTTTTAATTTATCTAAGCAGAGTTGTAGAGTCATGGTATAG
CATAGAGCCTAATTGGTATCTATATAGCCGTATGTGACAAATGCGTTAAGAAGCAGAAAA
AATACGTTTAGTGGCATTGTTTGGTGTGGTTAGCCGGGGACGCAGTTTTAGTTTCACGTG
GC