Aniseed - Construct

Construct 'CONS00001093'

Construct

ID

CONS00001093

Name

p.Cirobu.REG.KH2012.C9:4424417-4425395_μTcf7(b)>GFP

Min Promoter

Endogenous Promoter

Reporter

GFP

Comment

Reporter gene construct in which Wnttun5 mutated upstream regulatory sequence was fused to the green fluorescent protein (GFP) reporter gene.

This construct is testing Regulatory Region

REG00001124: Cirobu.REG.KH2012.C9:4424417-4425395_μTcf7(b)|Wnttun5

Sequence

Show sequence

Length: 920

CGTCGCCCAATCGCTGCCGTAATTACAGGTCCACACGCGAGCAGCTTGTTTGTTTATCTC
TGCATTTGCGAGCTACAGGGGCTTTAATTGGCCCGCCGAGTTTTGTCGGCAGTTTGTTAT
TTCAAGGCCGATCTCCGCATACGGGGATGCGCAAGCGACTGATCTATTTGATAATTGGCG
GTTTCCTCGCCCCTAGTGCTGGGCAGGGGTTGGAATCGAGCAATTACGATTGCGATCGCC
GCCGACCACCTATTGGGAGGCCGAGACTCGTCCGGGTATATTAGGATCAGAAACTTCACC
TGTGACACTTCTGTCCATCACCTCTTTTAAGTGGCGGAGGTCATATGAAAGTTAGGGGTA
TTTTGAAACTACGTCAGTATCGACAATTAGGTACAACCGATGGGTCACCATAGGCGCTAG
CGAAAAACTTGAAGACATTTTCGTTTTGCCGTTGGCGATTGCCGTTCTCCCATTTAAAGC
TCTACCTCAGATGGCAGTGCAGAGAGTAGAGGTGATTACAAGAGGCAGCCACAGTCGAAA
CGATATTACTCAGTTCGTAAAATCGTGACGGATTTGATACGGATGTGATCAGCATTTGTT
TCTGATTGGAAAGCTATACGGCTGTTTTGATAATATGTACCTATAACCAACAGCAAAGTA
AACGTCTAATTTTAACAGCAGCAGGTTGTAGGTCTGGGTTCACGAATTTTTCCAGCCACT
GTCGCTAAACTAAATCAACTCAGAAGAAAGTTCGCACTTGCAGAGGCGAGAACAACCCGG
ACTAGGGCGCTACTTACGCCCGAAACTGGATGGACTGAACGATAACATCGAGTTTACGAG
ATTTTCCCGAATTCACACCTACACCAGCGCCAGCGTCGCCACAGGACATCAGAGAAAAGG
TTTGAGCACAGGGGCGCTGG

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045

Show in situ data