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CONSTRUCT

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  1. Construct 'CONS00000193' > 
  2. Construct 'CONS00000984' > 
  3. Construct 'CONS00000985' > 
  4. Construct 'CONS00001274' > 
  5. Construct 'CONS00000986'

Construct

ID

CONS00000986

Name

pCirobu.REG.KH2013:KH.C3.2806725-2807227>NLSLacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

The regulatory region contains 447-bp 5' flanking region, 5' UTR and 5-bp coding region. The DNA fragment was fused in frame with the translated region of lacZ.

This construct is testing Regulatory Region

REG00001016: Cirobu.REG.KH2013:KH.C3.2806725-2807227

Sequence

Show sequence

Length: 503

AGCAATGTTTGACTTTCGTCGCTGCAATTGCAATATTGCACACGGACACAATACCGTTAT
CACCAATACTCTGCAAATCGTGGGCGCCAAGCTAATAAGCAGATAAGGGACGAGTGCGAG
CATAAATATTATGATAAGCTGGACTCTCGATTGGTCAGACGAGAAATAGCGAACAGCGAC
CGAAAAAAAGAGAAGAAGTCCGTAATTTGTATGGCTTTATAGCGGTCGACTCTCTACCGC
AAATCAGTGTATCTCGGTTCGCAGAGTAAGCAGCTTACAGTTTCGTGAGACCGAATTCAA
CGCAGTTCGATTAAATAAGTTGATAGCGTCTAATCGTTTGTATTTTATAATACCACAAGC
TCAGTAAGAATAAATGTCAACCAGTTTGATTTTTAGAAGTAAAGGCTTATAGTATTTCAG
TTATCTAAATATGTGTTTACTTTTCAGATCCTAAAAATAGTATAAAATTGAAAGCATAGT
TTTATATTAAAACGAAGAATGGC

Reference(s)

Horikawa et al. (2013). Transcriptional regulation in the early ectodermal lineage of ascidian embryos. Dev. Growth Differ. 55(9):776-85

Show in situ data