Login
Help

CONSTRUCT

Submit your Data

  1. Construct 'CONS00001107'

Construct

ID

CONS00001107

Name

p.Cirobu.REG.KH2012.C2.4475337-4475581_(Cirobu.REG.KH2012.S654:27045-27068)x4>GFP

Min Promoter

Endogenous Promoter

Reporter

GFP

Comment

A repressive element in the upstream sequence of Tbx6-b (S654.3) is inserted 4 times in the upstream enhancer of FGF9/16/20, 33pb upstream of the TSS. Silencer sequence is shown in capital letters.

This construct is testing Regulatory Region

REG00001080: Cirobu.REG.KH2012.C2.4475337-4475581|Fgf9/16/20

REG00001305: Cirobu.REG.KH2012.S654:27045-27067|Tbx6.b

Sequence

Show sequence

Length: 337

GTTGTTTACAGCAATTAAGCCGCCATATAGATAACTGTGCGCACTGGTCGTCTGCCCATT
TTTAGATAGCCTTCCCAGTGCTTCTGTTCTCGAGATTACGATCACAAGCGTAAGACGTCA
AGTTCACCGACAAAGATAAGAATCGCGACCCACACAAGTCACTGCCGCCCCTTCTTCGTT
TCTCGTCTAGACACGAATCAGCAGGAGAGCTAGACACGAATCAGCAGGAGAGCTAGACAC
GAATCAGCAGGAGAGCTAGACACGAATCAGCAGGAGAGCGTACACAAAAGCAGCGGGAAT
CTTGACTCGGAGCATCACTCTTGAATCGCCACTCAGC

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045

Show in situ data