Aniseed - Construct

Construct 'CONS00001200'

Construct

ID

CONS00001200

Name

p.Cirobu.REG.KH2012.C4.4315574-4315776>LacZ

Min Promoter

pBra

Reporter

LacZ

Comment

Construct containing (4315574-4315776bp) two putative Smad binding element and one binding element for Smad4 (a co-factor of regulatory Smad proteins) within the 100-bp upstream region of the the a-element of Otx driven by Brachyury promoter and with LacZ as reprter gene. The sequence was Blased in ANISEED based on the sequence in the supplementary sequence of Ohta 2013 article.

This construct is testing Regulatory Region

REG00001207: Cirobu.REG.KH2012.C4.4315574-4315776

Sequence

Show sequence

Length: 203

CGCGGCAGGTCGTCTTTTACCGAACATGTTTTCGGCGTGTTATCATAGCGAAGCACGCTG
CGTTTCCTTCACCTTACGACGTGTGATAGTGCGTGATAACTCGTATCCTTTCGCACTGCG
TTCCTATCCGTACTTTGGATCTGAAGCTCGTTATCTCTAACGGAAGTTTTCGAAAAGGAA
ATTGTTCAATATCTAAGATAGGA

Reference(s)

Ohta et al. (2013). Multiple signaling pathways coordinate to induce a threshold response in a chordate embryo. PLoS Genet. 9(10):e1003818

Show in situ data