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CONSTRUCT

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  1. Construct 'CONS00001301' > 
  2. Construct 'CONS00001570' > 
  3. Construct 'CONS00000844' > 
  4. Construct 'CONS00000839' > 
  5. Construct 'CONS00001191'

Construct

ID

CONS00001191

Name

p.Cirobu.REG.KH2012.L152:106567-108602::106013-106494>YFP-caax

Min Promoter

Endogenous Promoter

Reporter

YFP

Comment

Islet regulatory sequence drives YFP expression in the palps, and also sometimes in the notochord and the pigmented otolith, which are also normal sites of Islet expression. Here is used YFP-caax, a fusion protein for membrane localisation.

This construct is testing Regulatory Region

REG00001203: Cirobu.REG.KH2012.L152:106567-108602::106013-106494|islet

Sequence

Show sequence

Length: 2,534

GGCGCGCCGCCTCGCTTAATTGCGGTAAGTTTGTGGGTTGTTTAATAAAGTAGGGGGGTT
TGGGTTCGAAATCAGGCCAGGGTTGAAAATCCAGGGATCCCGGGTTCTAATCCAGGTCAG
GGTTGGAGAACCAGGGATCCTGGGTTCGAATTCAGGCCAAGAACTAGCATTGTACGCGTA
AGTGTCTTGAACAAAATTGCGGGTAAGTGCTTAAGCAGGAAAGGCAACAATTAACCTTAG
GATTCAGTATATAGTGCGAAGACGGATACATTATTAATGGGGGGGCGACCTTTGTAAGCG
CCTAACATTGGACTGGGCCGGGGGGTTGGGGCGCGATGTTCGCGATGAAATTGGATTTTT
ATCTTTTGGCAACAAAATTACCGTTCGCGTAGATCGCCATCTTTAAACCGCGCGGCGAAT
ATCTCAAGCCATTGTCACGTCACAATCGGCATTTATTATTTATTTACGTCACAACGGCTT
AATCTGAAGCTGCAAAAGGTATTTGTGACATCACAAAACATAACAATGAAGTTGTGTTAA
AGCATTGTGAATCACAAATACGAGATAATATACATCGCTTATGTCGTCATAACCGCGTCG
ATGCAGCCGCGTAAATCGCACTCAATGTCCCATTCAAGCTGCTACGTCACAATCGCTATT
CTTTCCCCACAATAGACAATGTTAGTTAACAATTTCCCGTGTGTGACGCAACAATTAAAA
CAATAAAGAACAATGCAGTGTATTCTAATACATTGTAGAATGCTCGCGTCATCTTACAGC
GCACACCGCGGTTGCATCGAATGAATAATGCAACAACATATCGACCCGCATTCGATAAAA
TATCAGACTTTTATATTTTCCTTAAAGACCTCAAGTATTTTAACTTTTTTTCGAAAATCT
TTTTTATTTTAATAATATTTTCGCAGTGACGTCATTAATTTATGACATCACCAGTGATAT
CATGACGTCACTCGATCAATATTAGCCAAGAGCAAAAATAGTGGATATTTTTCATTAATT
CTTTATTTGTTTATTTAACCGGAAACAAAATGGCCGCCGGTGACCTGGTTGATTGTGACG
TATATAACACGCAACACACGACTATTATGACGAAACACAGACTTGAATTGAAAATCTAGA
ACCCCGGTCACGTTATGGTAATGCTTATGACGAAATAATTGAAAGTTTTTGCGATTGTGA
CGTCATAATACCTTTATTGTTAGGCGATTGGTTAATTAGGACGCGTGTTCGTTTTTAATT
GTGACGTAATAAACATGAAATTAAACGAGATTGCCAAAGTTAAATGATGCATTATGACAT
CATAATATATAACTTTTATGTGATTCTAGAAAAGACGAAACCGCGAAAGTAGAACAAGAC
GATTGTTAACAAAGCTACGTCATAATCCATAATCAACACGCGAATCGCGTCGCTAAGTAT
CCGCACCTGTGACGTAACAATACTAAGACACAGGCAAACTTCGATATCGCTAAAATATAT
GATTTCTATGCGATCGCGATTAAGTGGTTTTAATTGTTCGCTAGCAAAGTGGTTTGTGGA
GATTAATTTGAGGTAATGTAGTTTAGAACCAACTTATTGTAAATTATCTAAAGTGGTCAC
ATGTATACGCACGTGTATATGGATTAATTAAGGGGGGTCATTAAGTGTAAAGTCGCTAAT
CCCCCTCACCCGTGAGACTTTAGTCATTAACAAAGTGTTTGTTAATTGATCGACAAAGTG
GGTCGCGGTGTAATAGTTGCTGCATTATGACATAACATGCATTATAAATGTGACGTCACA
TTGAATAAACAATCGTCTATATTTGGATTTTAGGACTTGAGATAAAAAAGTTAAAATATG
TTTGGGTCTTGATGGCTTGTAACTTTTGTTGGGGATTATTTTGTTAATTGTTGGGAGGGT
CAGGTTTTTAAGAGCTTTGTTAAGCGTTAAATAAAGAAAGAAAGAAATTTAAATAAATTA
ATTTAATTTTAGATTGAACTTTCTTAAACACAGAAATAAAATAAAGTTTTTGTTTGGCCT
CGAGGGTTAACTTAACATGGGCGTGTGAAATGTAATTAGTTAATGTAGTTAGTCGTATCT
GGATCGGAAGGCATTCATTTGCACTGTAGGCATTGAACTAGGAGGTTCACCACATCGAGG
AATATTTGGTCGCGCTGCGGGGATTGAGGGCCTTGTAGTTGGGGGGAGGTTTGAAAGTTT
CCCGAAGTAATATTAACTTTGTTGTATAGGACCCGTTGTTGCTGTTAGAGAGAATCGGAG
AAAAAGTTTCGAGAGAAGCTTTAGATAAATTGATTCTGTCGCATTCGAGTATTACAACTT
GTGTCTGTGACGTGAGCGAGTGAGGACAATTTACTGTGACGTCATAATAGCCAGCAATAT
AGGGGTGGAAGGTGAATACCCCGCTCTACAAGCGGAGAATTTCGAAATTTCAAACCTGGA
TCCCGGATACTCGCCGCCGTTGGCGGACGACTCAAGGTCGGGGTCGTTCACAAGTTTTAT
CAACGAAGCGCCGC

Reference(s)

Wagner et al. (2014). Islet is a key determinant of ascidian palp morphogenesis. Development 141(15):3084-92

Show in situ data