Aniseed - Construct

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Construct 'CONS00001302'

Construct

ID

CONS00001302

Name

p.Cirobu.REG.KH2012.C7:3853910-3854222_Bra2Fox2mut>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in the notochord despite the mutation of one brachyury and one Fox putative binding sites.

This construct is testing Regulatory Region

REG00001283: Cirobu.REG.KH2012.C7:3853910-3854222_Bra2Fox2mut|Ephrin3

Sequence

Show sequence

Length: 313

GAAATCTAAATGTATTGAACGGTAAACGTCGTATTGAAAAATGAATCTCTATTTATAACT
CTCTACAGTATACGGAGAGTCCCCACCCATTAAACAAACGTAATATTAATTGTTAGATAG
ATATGATTCACACAGGATGTATTTGTGGTGTCTGGATTAACCCCCAGTAAGAATTCTCTG
CCATACAATAGCAGCGTGTTTGAATTACTGCTTGTTTTGGCAAGTTGATTAAGTCAGGTC
AATCTAGGTCAAGAACACACACACACTGTATGTATATACACAACACAGATGTAATCGGCC
CATTGTAGTATTG

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data