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CONSTRUCT

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  1. Transcript 'Boschl.CG.Botznik201...' > 
  2. Transcript 'Boleac.CG.SB_v3.S14....' > 
  3. Transcript 'Harore.CG.MTP2014.S2...' > 
  4. Construct 'CONS00000782' > 
  5. Construct 'CONS00001228'

Construct

ID

CONS00001228

Name

p.Cirobu.REG.KH2012.C6:403942-404043>LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

This cis-regulatory sequence of Onecut/HNF6 drives no LacZ activity in Ciona intestinalis tailbuds and larvae.
A human β-globin basal promoter is localized between the regulatory sequence and the LacZ reporter gene. (It corresponds to FC construct in Pezzotti et al. 2014).

This construct is testing Regulatory Region

REG00001226: Cirobu.REG.KH2012.C6:403942-404043|Onecut_HNF6

Sequence

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Length: 102

TGGGCCATCTGCTGCGTTTTTCTGTTCGGCTTATATACGAGTGGTTCGCGTCGGCGCGCG
GTTTGAGCAAAAACAATAGGACCGCTGTCGAAAACAAGGCGA

Reference(s)

Pezzotti et al. (2014). Auto and cross regulatory elements control Onecut expression in the ascidian nervous system. Dev. Biol. 390(2):273-87

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