Aniseed - Construct

Construct 'CONS00001207'

Construct

ID

CONS00001207

Name

p.Cirobu.REG.KH2012.S816:16094-18001>YFP:caax

Min Promoter

Endogenous Promoter

Reporter

YFP

Comment

2kb upstream ZicL drives YFP expression in the progenitors of the notochord and nerve cord. Here is used YFP-caax, a fusion protein for membrane localisation.

This construct is testing Regulatory Region

REG00001191: Cirobu.REG.KH2012.S816:16094-18001|Zicl

Sequence

Show sequence

Length: 1,908

TGCATAGGGACGATCAACCATTACATTAGAATACATATTGTAAGATGATGAACTTCTCTG
GTATGCTGACTGCCGTAAACTAATGACATCACGCTGATTGACCTTGGTATTTGTACCTGT
CGCGTGCTACGCCTTCCTTTCGTAACACAATGACTTAATTATCTTCTTTATCTTTATTTA
TTTGACTTCCGATCGCCCGTCGCTTTCCCATGCGCACGTGGTTCGATCCCCCCACCTCCG
ATATTTGCCTCGCGAAGGTAATTATCGTGTCGATCGCGCGTCTCACGCTACTTTTGGCAC
GCTTTCGCTCGTGCCTGTCGACGCAAGGTTTACGTATGGCACTTTCTCTACGTGATGTCA
ACAACAAGTCAACAAATTTACTCATCGTAGAGAATAATTGCAAAAGTAAAAAATGTTTTT
CGTTCGTTCGAAAATAATACAACACATTGTAGGGTGGGGGAGGTGTGACCCTTTTGATTC
TATCCCCATTGGTAACAAGCAAAGTACATTCAAACAATTATCAACCGTCTTCTCACGACT
TTGATAGACCTATGTTAATTTTTTTATACATGGTTAGAATATTGGGATATTATGTGCTAA
AGGGGTCCTGTCTCCCCCCACCCTACTATATAAAATTTCAAGCAAGATTGAAGTTAAGTA
TTTACGCAATATTGTGTCCCAAGCGTTGCTTGAAAATTCATGGAATTTAAATTTCAGAAA
CTCATTGCGTAATTAACCAAGTAGAATTACGATAATTACACGTATTGTTAAACTTCCTCT
ATAAAAACCCGTCATCATGACGTCATCACTCGACGACTAGTTCGACGTCAAAACGATTTA
GTCGAATAAAAAAATAATCCGTATCTGGAATACTCGATTTTCTGTTCAATTTTTATTATG
TTCTTGTTTGCAAAGTAAGTTTGATAGGTAATTTTTGATCAGCAAATTTAATAAAAACAA
GAAAACTGCGCTCTAATAAATATATATTATAACTTGATTCTAAAACAAGATACGTAATTG
CACATTATAAAACACTATAATCAAATATTAAACTGCAAACTTGGTCATTGTATAAGTTAT
AGCAATACAATTTATGTTCCATTTATTTGGGCTGAATCGTTTATGTCATTTCCGTTCACT
AAACTATAATCCTTTATAATCTTTACACACGCTACAATGACTCGAGACAAACCGATTTAT
AAATAATGATGACGTAACAATTAACAAAGGGTCTGGAATGTTGAATTCGCCATAACTTGC
TGTTATCGAGTTTATTATAAGATTATTTTTGAAATTTAAAAGGTTTAGTATTAAGTCAGG
ACTCAGGAGTATGAAAATGATAACTGACAGATAATAATAATTTTTATCAGGTCGGTAAGC
TAGCATTACTATATTGGAAAAACCTAAAGGGAATAAACAAGTAAAACACGCATTTTTTTT
TATTAAAATAATTGGGAATCGGTAATTTAAATCTGAAAAATTACTGTTAATTATGATCTG
GTAATAACTTTCAAAATATTAATAGACGTTTTCTATGTATCTTACTTGTGTAAATATTTT
TTACAACGTGTAAATTTGTTGTTGACATCACGTAGAGAAAGTGCCATACGTAAACCTTGC
GTCGACAGGCACGAGCGAAAGCGTGCCAAAAGTAGCGTGAGACGCGCGATCGACACGATA
ATTACCTTCGCGAGGCAAATATCGGAGGTGGGGGGATCGAACCACGTGCGCATGGGAAAG
CGACGGGCGATCGGAAGTCAAATAAATAAAGATAAAGAAGATAATTAGGTCATTGTGTTA
CGAAAGGAAGGCGTAGCACGCGACAGGTACAAATACCAAGGTCAATCAGCGTGATGTCAT
TAGTTCACGGCAGTCAGGATACCGAAGAATATTCCATCTTACAATATG

Reference(s)

Gainous et al. (2015). Diverse ETS transcription factors mediate FGF signaling in the Ciona anterior neural plate. Dev. Biol. 399(2):218-25

Show in situ data