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CONSTRUCT

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  1. Construct 'CONS00001217'

Construct

ID

CONS00001217

Name

p.Cirobu.REG.KH2012.C6:403974-404485>LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

This cis-regulatory sequence of Onecut/HNF6 drives LacZ activity in the ectopic mesenchyme in Ciona intestinalis tailbuds and larvae.
A human β-globin basal promoter is localized between the regulatory sequence and the LacZ reporter gene. (It corresponds to A construct in Pezzotti et al. 2014)

This construct is testing Regulatory Region

REG00001215: Cirobu.REG.KH2012.C6:403974-404485|Onecut_HNF6

Sequence

Show sequence

Length: 512

ATATACGAGTGGTTCGCGTCGGCGCGCGGTTTGAGCAAAAACAATAGGACCGCTGTCGAA
AACAAGGCGATTTCGCGGTGCTTTAATATAACGACATTATGCCCTTTTTAGAGTTGTATT
TTAAAGCTACTACAGCGAGTTTTTGTCGCATTGTGGTAAACGTAACCTATGTATGAAAAC
ATTTATCGCAAAATATAATAAAGCAAAAGTAACTTTACTATTACACAAATTAATGATACA
GACTTACCAATCGTAGGACTTTAGATTCATTTTCATCTTTTTAAAATTCTGTAAAAAAGG
GAAAAAATAAAACACTTTGATTTTTTTAAATGATACAATAAAAGTACATGGCGAAAGCGG
TCCCATTTCCGTGGGTAAAGGTCCTATTAATCGTTTCAACATGTGCCCGAATAAATGAAC
ATATGGCGTGGCATACGGACACGACGCTCGGCACAGACCCGGCTAATATACAATAGACAA
GACTATTTCCTTTATTGCCCTGCTACATCTCG