- Construct 'CONS00001217'
Construct
ID
CONS00001217
Name
p.Cirobu.REG.KH2012.C6:403974-404485>LacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
This cis-regulatory sequence of Onecut/HNF6 drives LacZ activity in the ectopic mesenchyme in Ciona intestinalis tailbuds and larvae.
A human β-globin basal promoter is localized between the regulatory sequence and the LacZ reporter gene. (It corresponds to A construct in Pezzotti et al. 2014)
ATATACGAGTGGTTCGCGTCGGCGCGCGGTTTGAGCAAAAACAATAGGACCGCTGTCGAA
AACAAGGCGATTTCGCGGTGCTTTAATATAACGACATTATGCCCTTTTTAGAGTTGTATT
TTAAAGCTACTACAGCGAGTTTTTGTCGCATTGTGGTAAACGTAACCTATGTATGAAAAC
ATTTATCGCAAAATATAATAAAGCAAAAGTAACTTTACTATTACACAAATTAATGATACA
GACTTACCAATCGTAGGACTTTAGATTCATTTTCATCTTTTTAAAATTCTGTAAAAAAGG
GAAAAAATAAAACACTTTGATTTTTTTAAATGATACAATAAAAGTACATGGCGAAAGCGG
TCCCATTTCCGTGGGTAAAGGTCCTATTAATCGTTTCAACATGTGCCCGAATAAATGAAC
ATATGGCGTGGCATACGGACACGACGCTCGGCACAGACCCGGCTAATATACAATAGACAA
GACTATTTCCTTTATTGCCCTGCTACATCTCG