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CONSTRUCT

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  1. Construct 'CONS00001212'

Construct

ID

CONS00001212

Name

pCirobu.REG.KH2012:KH.L50.21.31922-32313:prCirobu.Fog>NLSLacZ

Min Promoter

bpFOG

Reporter

LacZ

Comment

Construct of the sequence from 31922 to 32313 base pairs containing 5 Otx sites, 3 Fox sites and 3 SBEs binding sites to whom was added a FOG basal promoter driving LacZ as reporter gene. The sequence was blasted in ANISEED based on the sequence present in the article Roure 2014.

This construct is testing Regulatory Region

:

Sequence

Show sequence

Length: 392

ACTGTCGCGCTTTATTTTAGTTTGTTTTTAGTCGCGCGCCTTTCTTACTACAGCTCGTGT
TTCGGCCCCGCTACTAATCGCTGCTGAGTAGTCTGCCACTCTGCGCGGGGCAGGTGTTAT
CGTGGGCAGTGGAATATTCCCTTAAAGAGCTGAGTCCCAGTCGCTAGTGGATCATAACAC
AGTCGGTCCAGTGGCGTGCTCTCTCTATTTTACTTGGCTGCCATGCAAGTGGTAGACGGA
AGGATTAAAGGTTTGTTTGTGTCGATCGCTTTTAGCTCAGAGATGCATTGAGTCGTAGAC
TATAGGAGGATTATTACGAAACAATAGCGGCGCACGACCATGATTATTACGTCAGGATTA
ACAATTTAAAACGTCGCAAAAAAATCAGCAAA

Reference(s)

Roure et al. (2014). An otx/nodal regulatory signature for posterior neural development in ascidians. PLoS Genet. 10(8):e1004548

Show in situ data