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CONSTRUCT

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  1. Construct 'CONS00001214'

Construct

ID

CONS00001214

Name

p.Cirobu.REG.KH2012.C6:404848-405698>LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

This construct showed no activity in Ciona intestinalis tailbuds and larvae.
A human β-globin basal promoter is localized between the regulatory sequence and the LacZ reporter gene. (It corresponds to D construct in Pezzotti et al. 2014)

This construct is testing Regulatory Region

REG00001212: Cirobu.REG.KH2012.C6:404848-405698|Onecut_HNF6

Sequence

Show sequence

Length: 851

TCGACTACACCAGATGCTTAGTGGTAATTAAAGTTTTTTGTTTGTGGGAATTAAGCACAT
ATTGGTCCTCACCAAACAATATAGAATAGAGGTAACATATAGTAGGGTGGGGAAGATGGG
ACACCTATAGCACACAATATCCAAGTATCCTGATCGTGTTTTAAACAAATAACAACGGTC
TATGAGAGTCGTGAGAAGACATTTCTATAATTCATTAGATTTTCTTAGTTTGCTACCAAA
TGTTACGAAAAAATAAATTGATTAAGTGTCCCACCCTTCTATATGTCCATTCATTTTCTT
TTATAATCTTACCTGGTAGTAAATAAAGTGTAATTTTAGGAATATACAACAGTATTCTAA
AGCCTTATAAAAAGTGTTTAATTGTTAAGAACATGGTTGGGAGATATGGGATATAATTTT
CTAACGGCATCCATATCTCAACAAGTAAGACTTAGACATATAGAAGTATAGTTCTTGGTG
TATATGGTCTGGTTTTGTAGCGCTATAATATAAACTACGTTACTGAAATTAGATAAAGCT
AGTGAGAAACTAAATATTTGTTTTCAGTCGTTTTTTTGCTAGGGCAAATCACTTACATAC
CATTAATAAGTCGATAACACCGAAAACCTAGTTTAAAAATCAGAGTTTCTTTAGTAAAAA
TACCACATTTTAACCTGCTATAATTAATTTCTAAGCTAAAGAATTTTAACCTGCGTTTTT
TTGGTCTTAGATTCGAATCGTTACGAGCATGATAACATGGTATTTATAATACGCTCTGAA
TAATAGGGCTTATTACTATCGACCAATGATTGAAATGAAAGTATATGCATATTCGGTGTC
AACAGAACACG