- Construct 'CONS00001214'
Construct
ID
CONS00001214
Name
p.Cirobu.REG.KH2012.C6:404848-405698>LacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
This construct showed no activity in Ciona intestinalis tailbuds and larvae.
A human β-globin basal promoter is localized between the regulatory sequence and the LacZ reporter gene. (It corresponds to D construct in Pezzotti et al. 2014)
TCGACTACACCAGATGCTTAGTGGTAATTAAAGTTTTTTGTTTGTGGGAATTAAGCACAT
ATTGGTCCTCACCAAACAATATAGAATAGAGGTAACATATAGTAGGGTGGGGAAGATGGG
ACACCTATAGCACACAATATCCAAGTATCCTGATCGTGTTTTAAACAAATAACAACGGTC
TATGAGAGTCGTGAGAAGACATTTCTATAATTCATTAGATTTTCTTAGTTTGCTACCAAA
TGTTACGAAAAAATAAATTGATTAAGTGTCCCACCCTTCTATATGTCCATTCATTTTCTT
TTATAATCTTACCTGGTAGTAAATAAAGTGTAATTTTAGGAATATACAACAGTATTCTAA
AGCCTTATAAAAAGTGTTTAATTGTTAAGAACATGGTTGGGAGATATGGGATATAATTTT
CTAACGGCATCCATATCTCAACAAGTAAGACTTAGACATATAGAAGTATAGTTCTTGGTG
TATATGGTCTGGTTTTGTAGCGCTATAATATAAACTACGTTACTGAAATTAGATAAAGCT
AGTGAGAAACTAAATATTTGTTTTCAGTCGTTTTTTTGCTAGGGCAAATCACTTACATAC
CATTAATAAGTCGATAACACCGAAAACCTAGTTTAAAAATCAGAGTTTCTTTAGTAAAAA
TACCACATTTTAACCTGCTATAATTAATTTCTAAGCTAAAGAATTTTAACCTGCGTTTTT
TTGGTCTTAGATTCGAATCGTTACGAGCATGATAACATGGTATTTATAATACGCTCTGAA
TAATAGGGCTTATTACTATCGACCAATGATTGAAATGAAAGTATATGCATATTCGGTGTC
AACAGAACACG