- Construct 'CONS00001203'
Construct
ID
CONS00001203
Name
p.Cirobu.REG.KH2012.S544:4240-5239>H2B:CFP
Min Promoter
Endogenous Promoter
Reporter
Comment
The DMRT enhancer drives CFP expression throughout the anterior neural plate and adjacent anterior ectoderm. Here is used H2B:CFP, a fusion protein for nuclear localisation.
TAGTAGGGTGGAGGAAGATGGGACACCTTTGGCACATAATATCCAAATATCCTTACCCCG
TTTTACACAATTAACAGCGGTATATGGGAGTCGTAAGAATTCCGTTTTATAATTATTTGC
ATGTTCTTTGTTTGCTACTAAATTGGACGACAAAATGTAGTCAAAAGGTGTCTAATCTTC
CCCTACCCTACTATACGACTTGGGGCATAGTTATAAAGTTATTCCGTTTCATTTCTTGGC
GTTAACCCGACGATCAGTAATTAACGATATTCTTCATAAATAAATAATGCGAGAACTGCA
ACAGGACTAGGGAAGTTACTTAAGCGAGCATGTGATTAGAAATTGCCACTGAAGCACTTT
AAAATGCCGCCAAAACACATACACCTTCATGACACATTGTTTTACAGAATCTCAATTTAA
AACCCGTCGACAATTATCGGATTTCCGCCGCAGAGTCACAGGAAGTTGTTTTGCTTAATA
ATACTTGTTTTAGATAAGCTGTTATGTTGCTCAATGAATAACAATAATGTTTACGTTGGT
TGTTGTGGCAATGTACATTGTAACTTCCACAAACCTCTCAGAGGGCAAACACATTAAGCA
AACTGAATTTGCCAAGTACTTCGGGAAGTCACTGATAAGCGGACAATACGAAGCGATCAC
AGGGAATTGTCGCACGCCAGAATGTAAACAAAAACATCGCCTGTGCACCGCGCGATCGAA
GACACACAGCGCTGACTAATTGACCAACACAAAAGCGAGGAAAGAAAAAAAAGCGAAAAC
GAGAACGGGATATTAATTGTACACGATTGTGACGTAAATTCGAGGCATTTTTTCTTCCGA
AATCGAGCGGTTTAAATACCTCGCGTCTTTATTAGGTACAATCATATTGCCTTGATACCT
TGCTTAGAACAGTGAATACATATAAATCGTATTAAAAGCTTTAGAGTGTTAAACCAAAGA
GTTACAGAAATTATAGCAAACAGTTCGTTTAACTGGCATG