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CONSTRUCT

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  1. Construct 'CONS00000991' > 
  2. Construct 'CONS00001275' > 
  3. Construct 'CONS00000998' > 
  4. Construct 'CONS00000999'

Construct

ID

CONS00000999

Name

pCirobu.REG.KH2013:KH.C6.1850412-1851424>NLSLacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

The regulatory region contains 975-bp 5' flanking region, 5' UTR and 2-bp coding region. The DNA fragment was fused in frame with the translated region of lacZ.

This construct is testing Regulatory Region

REG00001030: Cirobu.REG.KH2013:KH.C6.1850412-1851424

Sequence

Show sequence

Length: 1,013

TATCTTCACGGTTCTAACACAGCGTTGTTTATTTCTTAAATCGCGATTGGGGATTATGGG
ATATTATGTGCCAACGGTATCCATGTTACCCAACAGTACTATATTAAATATTTTGATTTG
ATTTTGAGACTTTCTGCAAACTAAAGATTGCTTATCGTTTCCGTTACTTGACGTCATCAT
TTTGTTTCGTAAACAATAGAGATTGTGACGCCACAGATAACAATTGCAGAAACTCGCGAC
CGCGAATTGCATTATCTGATAATCACTTTCTATTATGACGTAACACTTGCAGATCGTAGA
GAAAAGAGGACGAGCTGGTTCTATTTAAATACAAAGCAAGTCGAAAGTGTTGGCAGCCAG
TGTATGAAGTAAGGATCAACTGTCTTATAGTAGGTTAAGGTAAAATATTCCCATGTTTTT
ATTCTCTTTTCTACGGCGCGCGCATTTGCACCAATGCTAAGGATGGGAGCATACAAGTTT
AGGTGTGAATAACATAATATATGGTGGGGTAAGATGGAACACGTTTTCATTCTCTTATTT
CGTCCTATTTGGTGGTAAACAAAGAATATTTAAGGAACTACATAACCGTATCCTCACCAC
TGTTAAGATCGTTGTTATTTGTTAAAAACGCGATTAGGAAATATGAGATATTATGTTCTA
ACGGTATCCATCTACCCGACCTACTACACGTTCTCCAACCCAGTATGCCCTACTACAGTG
CGTGAGATGATAAGACAGTAGGGCATACACCTGGTAACAGCGGGTGTTTAGTAAAGATCA
ACCTTTTTCCAATGCACGTTGTCTTAGTACAGGTTTATTTAGTTTTGCGTTTAGGAAATT
AGTTTGCAAGTGAAAAGCGCGTTGTTGTTTTAATGTATTTATTTTATCTTACTTTGAATT
GAGAAAAGGGTTATTTGTTGCAGATTTGTGTATATACTTTGTTATGGCATATATTTAAAA
CTGTGTTTCCTACAGGTTGTTTGAGTGCTAAGTAAAATTGTATAATTGGACAT

Reference(s)

Horikawa et al. (2013). Transcriptional regulation in the early ectodermal lineage of ascidian embryos. Dev. Growth Differ. 55(9):776-85

Show in situ data