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CONSTRUCT

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  1. Construct 'CONS00001234'

Construct

ID

CONS00001234

Name

p.Cirobu.REG.KH2012.C6:403782-404043(µ_Ngn-OCa)>LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

This mutated cis-regulatory sequence of Onecut/HNF6 drives LacZ activity in the ectopic mesenchyme in Ciona intestinalis tailbuds and larvae.
A human β-globin basal promoter is localized between the regulatory sequence and the LacZ reporter gene. (It corresponds to FG(Ngn-OCaMut) construct in Pezzotti et al. 2014).

This construct is testing Regulatory Region

REG00001232: Cirobu.REG.KH2012.C6:403782-404043(µ_Ngn-OCa)|Onecut_HNF6

Sequence

Show sequence

Length: 262

CTGACTAAACTGTTTTATAATTGAATATTTTTCTTTCCAAGGCTTGAACTTGTCTTAATT
GTGGGGGGTTGGAATTTAAACAAATTTTCGATCAGTCCTTTACGGTTGAGCTCCAAGCTC
GAATGGAAGACTGGTGCCGTACTCGTATGGGAGTAAGCAATGGGCGGTGGGGTGCGTTTT
TCTGTTCGGCTTATATACGAGTGGTTCGCGTCGGCGCGCGGTTTGAGCAAAAACAATAGG
ACCGCTGTCGAAAACAAGGCGA

Reference(s)

Pezzotti et al. (2014). Auto and cross regulatory elements control Onecut expression in the ascidian nervous system. Dev. Biol. 390(2):273-87

Stolfi et al. (2011). Neural tube patterning by Ephrin, FGF and Notch signaling relays. Development 138(24):5429-39

Show in situ data