Aniseed - Construct

Construct

ID

CONS00000998

Name

pCirobu.REG.KH2013:KH.C6.1850093-1851424>NLSLacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

The regulatory region contains 1964-bp 5' flanking region, 5' UTR and 2-bp coding region. The DNA fragment was fused in frame with the translated region of lacZ.

This construct is testing Regulatory Region

REG00001029: Cirobu.REG.KH2013:KH.C6.1850093-1851424

Sequence

Show sequence

Length: 1,332

TATCTATCGTCATGACCCATAAATTGGTTATTACTGTCAAATAGATAGCGTTTGATGTTA
CCAGTATTTTAGGTCGCTGCGGTATTTTCAACTGGTTAGTGGTGTTACACCAAATTCGCT
TTTTCGCGAAAACTGCTTTGGTTACTTCACGGTTCGATGCGTAAACGTAAATTATGGCTC
GTTACGTCACAATTGGCTTTGTTTACGTGGCCAAGCGGAAACGATGACGAAATTATATGA
AATTCATTTTCATTATTTTTTGTTAAAATTATAATTTTTCAGTGAAACTTTGTAGCTTTA
TTACAGAATTCTATATATGTATCTTCACGGTTCTAACACAGCGTTGTTTATTTCTTAAAT
CGCGATTGGGGATTATGGGATATTATGTGCCAACGGTATCCATGTTACCCAACAGTACTA
TATTAAATATTTTGATTTGATTTTGAGACTTTCTGCAAACTAAAGATTGCTTATCGTTTC
CGTTACTTGACGTCATCATTTTGTTTCGTAAACAATAGAGATTGTGACGCCACAGATAAC
AATTGCAGAAACTCGCGACCGCGAATTGCATTATCTGATAATCACTTTCTATTATGACGT
AACACTTGCAGATCGTAGAGAAAAGAGGACGAGCTGGTTCTATTTAAATACAAAGCAAGT
CGAAAGTGTTGGCAGCCAGTGTATGAAGTAAGGATCAACTGTCTTATAGTAGGTTAAGGT
AAAATATTCCCATGTTTTTATTCTCTTTTCTACGGCGCGCGCATTTGCACCAATGCTAAG
GATGGGAGCATACAAGTTTAGGTGTGAATAACATAATATATGGTGGGGTAAGATGGAACA
CGTTTTCATTCTCTTATTTCGTCCTATTTGGTGGTAAACAAAGAATATTTAAGGAACTAC
ATAACCGTATCCTCACCACTGTTAAGATCGTTGTTATTTGTTAAAAACGCGATTAGGAAA
TATGAGATATTATGTTCTAACGGTATCCATCTACCCGACCTACTACACGTTCTCCAACCC
AGTATGCCCTACTACAGTGCGTGAGATGATAAGACAGTAGGGCATACACCTGGTAACAGC
GGGTGTTTAGTAAAGATCAACCTTTTTCCAATGCACGTTGTCTTAGTACAGGTTTATTTA
GTTTTGCGTTTAGGAAATTAGTTTGCAAGTGAAAAGCGCGTTGTTGTTTTAATGTATTTA
TTTTATCTTACTTTGAATTGAGAAAAGGGTTATTTGTTGCAGATTTGTGTATATACTTTG
TTATGGCATATATTTAAAACTGTGTTTCCTACAGGTTGTTTGAGTGCTAAGTAAAATTGT
ATAATTGGACAT

Reference(s)

Horikawa et al. (2013). Transcriptional regulation in the early ectodermal lineage of ascidian embryos. Dev. Growth Differ. 55(9):776-85

Show in situ data