- Construct 'CONS00001144'
Construct
ID
CONS00001144
Name
Brachyury>mCherry
Min Promoter
Endogenous Promoter
Reporter
Comment
Region from -483 bp upstream the +1 to the first 17 codons is the minimal enhancer which drives the entire Ci-Bra expression (the transcription start site is the nucleotide +1). This region contains three essential cis-regulatory elements and is placed upstream the reporter mCherry.
Show sequence
Length: 1,557
GACGGGGTAGCAGTTATTGAAGTAGACGGACAACCCCTGCCGGGGAATTACCTGCATAGT
ACTTAAGCTCTTATGACGTAATAATGGACCTTACAAAAGAACAAACAATTAATATATAAG
AATTCGGCTTATGACGAAATAATGTAAACGTCAAATTATTGTGACATTTATTATTGCGTC
ATTGAGGTTTTGTCGCCCACTCGCGAAACGTCAGGCAATTAGTTTCCTTGTTTACGGAAC
AAACGACTGCGGGGGTGCGAATTGTGACGTCATCAATCAAACTAAAAAGGGGAGTTGTGA
CGCAATAATAAAGTAATTTTCAAATGCGATTGTTATGTAATAAAAAAGGAAAAAATAATG
AACATAATTATTCGTTTCTTCGACTGCGACAGCGAAGATAAAATTAATTAAAACAACAAA
GAGAACGAAATTTGCAACAAGACGACAGTCGATAAAACACGATGAGTAGAAACTACTTGA
GTAAAAGTGTCAAATAAAACAAAAAATGAAAAAAACACACCCAACGTACAATAAAACTTA
CGGCAAATTGTAGTTAAAATTCATAATTACAAAACAATAAAGATCATATAAACAAAATAT
TAAAGATCATATTAACAATATAATATAGATCATATTAATAGCGACAAACCTTATCTGGTG
TTACGTCACAATACAAACAAAATATTTCGACATGTCAATCAAAATCGGAAACCAAGTTTC
AACTTCCCACGCAAGACAATGGGAAAGTAACACGTCACAATACACTTGGTGACGTCATAT
CACTAAAACAAACACAAGGTGTTCGATCCAGCTGTGAAAGTAAACATAGAGCGCCACCAC
ACGAGCAACCCTCACAGCTGGATGCCACCACCTACGGCGCACTTTCAACAAACATAAAAT
TTCAAAAAGAAGAAGTTATGACGTCACAATCCTGTATAAACTTGCACCCGAGTGTGATTT
GGAGGCAGAATGTTTTCGAAGCTCAGTGCGAGTTACAAACCTATAATGACGTCATCAGAT
AGTAAGTTAGCAGGTATGACGTCATCAGAATCAATTGAAACATGTGCAGTAAAGGTGAGT
TGCATGTTGTGTAAGGTTGATCTTAAATTAATAATATTCCTCCGGTAGATGGCGCTAATA
GAGCATGGTTTATGGTCGAGGTTTCACGCGTTTGTCAACGAGATGATTGTGACAAAAAAT
GGACGACGAATGTTTCCGGTTCTTAAAACATCGATTACGGGACTTGATCCAACTGCGATG
TATTCCGTCATGCTTGACTTTGTACCCGTGGATAACAATAGGTAAGTTTATGACGTCATA
ATGTGGTTGATTGTAAACATTGTTATTGCTAGATGGAAATATGTGAACGGTGAGTGGATC
CCCGGGGGAAAACCCGAACCCCATGTTTCGTCATGTGCTTATATTCACCCCGATTCACCC
AACTTTGGTTCGCACTGGATGAAACAACCCGTTGGTTTCAGTCGCGTCAAACTTACTAAT
AAAGCTACAGGGAACCCCCAGGTACGTAAGCAAGTGACGTCATTATACTAGTGACGT
