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CONSTRUCT

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  1. Construct 'CONS00001208' > 
  2. Construct 'CONS00000772' > 
  3. Construct 'CONS00000791' > 
  4. Construct 'CONS00000629' > 
  5. Construct 'CONS00001152'

Construct

ID

CONS00001152

Name

Ci-VGAT CNR+fkh Kaede

Min Promoter

Cirobu.REG00001172.KH2012.C11.4405380-4405537

Reporter

Kaede

Comment

The CNR region from ci-VIAAT (VGAT) was added at the minimal promoter fkh/FoxAa (from -158 to +20 relative to the translation start site, Di Gregorio et al. 2001) and upstream the coding sequence of Kaede reporter gene.

This construct is testing Regulatory Region

:

Sequence

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Length: 378

TTTCGCAGTAGAAAAAACGTTGTACAAGAAATTCAGTAATTCCGGAAAAGACTTTTAGTG
AATCTGCAGCGCATTCCAAATTATTGTAGGTGAATCGGCTGCAGTTTGATTTCGCTTGAT
TGTCCATGACACGTAGTTTAAATAAGTTGCCAAGGATCGATTCCGGAAAAAAAAACATTT
GGTGATTCTGCAGCGCACTAGACCAATAATTTCGCCGCCATTTTTTTCTTAAAATATCAC
GGCCAGTTTTTAACAAGTTATTTTTCTAGCTTTCTATTTGAAATATTATACACAACTGCT
GCTGTAATTTCTGAGAAAGCTTTTGAAATCGGATTACTTTTGCAAGTACAAAAATGATGT
TGTCGTCTCCACCGTCAA

Reference(s)

Nishitsuji et al. (2012). Cell lineage and cis-regulation for a unique GABAergic/glycinergic neuron type in the larval nerve cord of the ascidian Ciona intestinalis. Dev. Growth Differ. 54(2):177-86

Show in situ data