- Transcript 'KH2012:KH.L133.6.v1....' >
- Transcript 'KH2012:KH.C9.371.v1....' >
- Transcript 'Mooccu.CG.ELv1_2.S70...' >
- Construct 'CONS00001587' >
- Construct 'CONS00000655'
Construct
ID
CONS00000655
Name
pCi-Pitx -2735/-2540 Second Otx site mutated:CES2:LacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
Minimal enhancer upstream of Pitx gene from -2735 to -2540, called D1bcde in the paper. The Pax binding site is absent. The second Otx motif was mutated (TAATC => TCATC). The mutation (m5/6 in the paper) affects the expression in the neurohypophysis primordium.
KH transcript model was taken as reference for the region s coordinates.
The sequence is based on the first codon on the KH transcript.
ACGGATTAAGGTAATATTTCTTTTTATGTTGCCGCCCAGTCGGTTCCTGACAATACACTG
ACAGGTAGTGCGAATAAAAGGCGCTCGTGGATGTAAAAAAACACTAATAATCTTCAGTAA
TTTCCGTGCCTTTCCTATGGGCGAAAACTCCCGCCGTGTGAAACGACTTCAAAAGGCGGA
CAGTCGCCGCCAAGG