Aniseed - Construct

Construct 'CONS00000667'

Construct

ID

CONS00000667

Name

pCi-Pitx -2751/-2696 repeated x2 Second Forkhead site mutated:CES2:LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

Minimal enhancer upstream of Pitx gene from -2751 to -2696, called D1(ab)(ab-Tmut) in the paper. The region named D1ab in the paper was repeat 2 times and the forkhead site in the repeated region was mutated. This region can t drive expression in the neurohypophysis primordium.
The forkhead mutated site was replaced by "N". The sequence is based on the first codon on the KH transcript.

This construct is testing Regulatory Region

REG00000698: Ci-Pitx -2751/-2696 repeated x2 Second Forkhead site mutated

Sequence

Show sequence

Length: 110

AAACGCGACGACCTCCACGGATTAAGGTAATATTTCTTTTTATGTTGCCGCCCAGAAACG
CGACGACCTCCACGGATTAAGGNNNNNNNNNNNNTTATGTTGCCGCCCAG

Reference(s)

Show in situ data