- Construct 'CONS00000782'
Construct
ID
CONS00000782
Name
pCi-Noto8 -3248/-218 delta -1143/-2147::LacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
construct (named p(-3.0 k)(delta 2.0k-0.8k)Noto8/lacZ in the paper) contains the region upstream of Ci-Noto8 in front of a LacZ reporter gene. In this construct a deletion was performed and had no effect on notochord expression.
CCTTGAGACATATTTCCTTTGCAGCGTCCTTCTTATAATATATGCTACGGTGTATTTGAT
GAATAACTGCAAAAACACTAGAGCGACCAATGACGCTACCATTTTATATGACAACAACGA
GAGGAAGTAACGTCATAGCGCTGAAACATCCGCAACATTCCACTGTGGTGTTAAAACCAG
AGTGTGATTTTTAAACATTAGATTTAGTGAACCAAGTTATAAAAGCTTTTCGCGTAAATT
GTCTTGCAACAACTGTCATTTAGGTTGGTTTACGATAGGAAGTTCTTATAATTCATATGC
TTATTTGGCATTTGCACAATTATAGAGCTTACAAATTATAATAATAAGGACGACTTTGGT
GGTAGCAGTTGTAGTCTCCACTAAGTATAGCTTTGCAATTGTTCGAGAAATAATAGTGTC
GTCGTTGCGGTGTGTTATACGTTCAAATTAATCCAGTGCGTACTTTTGATTTGTATTGTT
TGTTAGTATCGTAAAACTAAAATACAAGAATTACAATACAAAAAAAATGCAAATATGCCA
TGTAACAAGAGCAACAGAAAGTAAAATATATGTAGAATTGTGAAGCATTACAGCAGGTAT
ATCAAACAAATTTAGTAGGGTGAATATGGGACACCTTTGTGTTTCATTTTATCGTCCCAT
TTGGTAGTAAACAAAGAACATTCAAAGAATTATAAAACCGTATCCTTTGGGCTCCCATAG
ACCGTGTTGTAATATGTTTAAAACTCGATCAGGATATTTGGATATTACGTGTTAAAGGTG
TCCCATCTTCCCCCACCCTACTATATATAGGTTATGCATTCACATTTATTTGACAGACAG
TAATTCGTGCTAACTAGGTTACATTAGGTTAGCTGAACACAATTGTGTAACTGGAAGATC
AAATGCCGGGGTAAGATTGACCAAT-----------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
---------CGTTGAACTTGTGGGGTAAAGATGGGTCGCTCGGTCTTGCTGTTGCGTGAA
CGTAATCTGGAGCTTTAGGCAAGCACATCATGTAGCCGATCTTTGCTTTTGTTTTAGCAT
TTGCCGAAAACGAAGATAATTCAAAACTCCCTCCGCTGGTCAGCGGTTTATCTGTTGATG
GATTTGTTTACTAGATATATATACTTACACTTTAATGATATATACTTATACTAACTTAAA
CGTAGACAACTTACTTGAATTGACCTCGCTTATAACTACAAGCAACATGAACCAAATGCA
CTTCATAATGACCAATGTTAATAAACCTAACCCCACAGAAATCTGTGTTGTGTTAAAGTG
GGTAGTTAATTAAAACAGCTTAGCGAACAATGCTGTCTGAACAATAAATAATGAATGCAT
ACCACAGGTATTGGATTGGTGTTTTGGCACGTTTTATGCAACACATCTTATAGTAGCAGT
ATATAGCAAACAGATTTACTTTATTTGGGGCCGTATTTTAAGATTAAAATACATCATGAT
TTACCCATGATATGTATAGTATTAACTAAACTTCTGCTCATTTAAATCAACCAAACTCTA
ACGAATACACAATCTAAACAACATGAGAGTCAAACTATATTGAACGGGCACGTATTATTA
CAAAAATAATTTATGTAGAAGATGCGGAAAAAACGGTACAACCTTCTGCCGAACTGTGAC
CTCAAAATATTAGCCTGGTCGTTCTGTATTTCAGTCAAAATATTGTTCTGCTGTTGTCCC
GAACATTCGTTTCCATGTTTTACAAAATGCGGGTCATGATTAAATAGAAAAAAATACACG
TGCATGTTGTCATCATACATTTACTTGGAACCGCAAAAACATATGTTTTGGTATTTAGGT
TAAAATCAACAGCTAATGCTATATATTGCTAAACAACGTAATTGCTGAAACCACAGATAC
ATAAACATATAGTGGGGTGGGGGAAGATGGGACACTTTTTAACTCTATTTACTCGTCCCA
GTTAGTAGTAAACAAAGAACATTCAAAGAATTATAATATATCCTTACGACTTCCATGAAC
CGTTGTTAATTGTTTAAAACACGATCAGTG