Login
Help

CONSTRUCT

Submit your Data

  1. Construct 'CONS00000782'

Construct

ID

CONS00000782

Name

pCi-Noto8 -3248/-218 delta -1143/-2147::LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

construct (named p(-3.0 k)(delta 2.0k-0.8k)Noto8/lacZ in the paper) contains the region upstream of Ci-Noto8 in front of a LacZ reporter gene. In this construct a deletion was performed and had no effect on notochord expression.

This construct is testing Regulatory Region

REG00000819: Ci-Noto8 -3248/-218 delta -1143/-2147

Sequence

Show sequence

Length: 3,030

CCTTGAGACATATTTCCTTTGCAGCGTCCTTCTTATAATATATGCTACGGTGTATTTGAT
GAATAACTGCAAAAACACTAGAGCGACCAATGACGCTACCATTTTATATGACAACAACGA
GAGGAAGTAACGTCATAGCGCTGAAACATCCGCAACATTCCACTGTGGTGTTAAAACCAG
AGTGTGATTTTTAAACATTAGATTTAGTGAACCAAGTTATAAAAGCTTTTCGCGTAAATT
GTCTTGCAACAACTGTCATTTAGGTTGGTTTACGATAGGAAGTTCTTATAATTCATATGC
TTATTTGGCATTTGCACAATTATAGAGCTTACAAATTATAATAATAAGGACGACTTTGGT
GGTAGCAGTTGTAGTCTCCACTAAGTATAGCTTTGCAATTGTTCGAGAAATAATAGTGTC
GTCGTTGCGGTGTGTTATACGTTCAAATTAATCCAGTGCGTACTTTTGATTTGTATTGTT
TGTTAGTATCGTAAAACTAAAATACAAGAATTACAATACAAAAAAAATGCAAATATGCCA
TGTAACAAGAGCAACAGAAAGTAAAATATATGTAGAATTGTGAAGCATTACAGCAGGTAT
ATCAAACAAATTTAGTAGGGTGAATATGGGACACCTTTGTGTTTCATTTTATCGTCCCAT
TTGGTAGTAAACAAAGAACATTCAAAGAATTATAAAACCGTATCCTTTGGGCTCCCATAG
ACCGTGTTGTAATATGTTTAAAACTCGATCAGGATATTTGGATATTACGTGTTAAAGGTG
TCCCATCTTCCCCCACCCTACTATATATAGGTTATGCATTCACATTTATTTGACAGACAG
TAATTCGTGCTAACTAGGTTACATTAGGTTAGCTGAACACAATTGTGTAACTGGAAGATC
AAATGCCGGGGTAAGATTGACCAAT-----------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
------------------------------------------------------------
---------CGTTGAACTTGTGGGGTAAAGATGGGTCGCTCGGTCTTGCTGTTGCGTGAA
CGTAATCTGGAGCTTTAGGCAAGCACATCATGTAGCCGATCTTTGCTTTTGTTTTAGCAT
TTGCCGAAAACGAAGATAATTCAAAACTCCCTCCGCTGGTCAGCGGTTTATCTGTTGATG
GATTTGTTTACTAGATATATATACTTACACTTTAATGATATATACTTATACTAACTTAAA
CGTAGACAACTTACTTGAATTGACCTCGCTTATAACTACAAGCAACATGAACCAAATGCA
CTTCATAATGACCAATGTTAATAAACCTAACCCCACAGAAATCTGTGTTGTGTTAAAGTG
GGTAGTTAATTAAAACAGCTTAGCGAACAATGCTGTCTGAACAATAAATAATGAATGCAT
ACCACAGGTATTGGATTGGTGTTTTGGCACGTTTTATGCAACACATCTTATAGTAGCAGT
ATATAGCAAACAGATTTACTTTATTTGGGGCCGTATTTTAAGATTAAAATACATCATGAT
TTACCCATGATATGTATAGTATTAACTAAACTTCTGCTCATTTAAATCAACCAAACTCTA
ACGAATACACAATCTAAACAACATGAGAGTCAAACTATATTGAACGGGCACGTATTATTA
CAAAAATAATTTATGTAGAAGATGCGGAAAAAACGGTACAACCTTCTGCCGAACTGTGAC
CTCAAAATATTAGCCTGGTCGTTCTGTATTTCAGTCAAAATATTGTTCTGCTGTTGTCCC
GAACATTCGTTTCCATGTTTTACAAAATGCGGGTCATGATTAAATAGAAAAAAATACACG
TGCATGTTGTCATCATACATTTACTTGGAACCGCAAAAACATATGTTTTGGTATTTAGGT
TAAAATCAACAGCTAATGCTATATATTGCTAAACAACGTAATTGCTGAAACCACAGATAC
ATAAACATATAGTGGGGTGGGGGAAGATGGGACACTTTTTAACTCTATTTACTCGTCCCA
GTTAGTAGTAAACAAAGAACATTCAAAGAATTATAATATATCCTTACGACTTCCATGAAC
CGTTGTTAATTGTTTAAAACACGATCAGTG

Reference(s)