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CONSTRUCT

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  1. Construct 'CONS00000746'

Construct

ID

CONS00000746

Name

pCi-Nut1 -426/94 pVenus::EGFP

Min Promoter

bpVenus

Reporter

GFP

Comment

Construct containing the region upstream of Ci-Nut1 from -426bp to 94bp, and inserted into the promoter of Venus reporter gene (construct named -422bp in the paper).

Primers used for the construct : Forward primer Nut1UPSU-422/SalI 5-ACGCGTCGACCACACACACAGAAAATCGG-3 and the reverse primer Nut1UPSL/BamHI 5-CGCGGATCCTCCAAAATCAATTTCCATG-3.

This construct is testing Regulatory Region

REG00000783: Ci-Nut1 -426/94

Sequence

Show sequence

Length: 520

GATTTCAGAACACACACACAGAAAATCGGTCGTGCCTGCTGCTGCAACTAGTGCGACACC
TCAATAATTATGAGACTGCCTCAGTGCATTCCTATCAACGTGTTTTGCGCTCGTATCACC
CAAAAAGCGTGCCTCGTGCTGCCTACACGGTGTGGCTACATAAAAACGTCGCGTGTTTTA
CTGTTCCAGTTCGTTTTTGTATTCGTGGAAATAGGTTCGCATTTTTTATTAAGAAAGAGT
TTGGTTTTCGAGGTCACTGAATTGCAATTAGAAGGCATTTAATAGTAGCACTAGGACACT
CTATTCACTGCGTTGTCTAAATCCATGCGAAACAACAAAATAAGTCGCAAGACATGCTGT
GCGTGTATTGTTTTTTTGAAACCCCGTCGCTTTGTGAAAATCTGGTTGATTATTTTTTCG
TACCAGTTTTACAGTTTAAATACGACTGTGCTTCAGTTTTTGTTAGTATTGAGTTGTACA
CTATATCAACAACATGGAAATTGATTTTGGATTTGCAAGA

Reference(s)