- Construct 'CONS00000746'
Construct
ID
CONS00000746
Name
pCi-Nut1 -426/94 pVenus::EGFP
Min Promoter
Reporter
Comment
Construct containing the region upstream of Ci-Nut1 from -426bp to 94bp, and inserted into the promoter of Venus reporter gene (construct named -422bp in the paper).
Primers used for the construct : Forward primer Nut1UPSU-422/SalI 5-ACGCGTCGACCACACACACAGAAAATCGG-3 and the reverse primer Nut1UPSL/BamHI 5-CGCGGATCCTCCAAAATCAATTTCCATG-3.
GATTTCAGAACACACACACAGAAAATCGGTCGTGCCTGCTGCTGCAACTAGTGCGACACC
TCAATAATTATGAGACTGCCTCAGTGCATTCCTATCAACGTGTTTTGCGCTCGTATCACC
CAAAAAGCGTGCCTCGTGCTGCCTACACGGTGTGGCTACATAAAAACGTCGCGTGTTTTA
CTGTTCCAGTTCGTTTTTGTATTCGTGGAAATAGGTTCGCATTTTTTATTAAGAAAGAGT
TTGGTTTTCGAGGTCACTGAATTGCAATTAGAAGGCATTTAATAGTAGCACTAGGACACT
CTATTCACTGCGTTGTCTAAATCCATGCGAAACAACAAAATAAGTCGCAAGACATGCTGT
GCGTGTATTGTTTTTTTGAAACCCCGTCGCTTTGTGAAAATCTGGTTGATTATTTTTTCG
TACCAGTTTTACAGTTTAAATACGACTGTGCTTCAGTTTTTGTTAGTATTGAGTTGTACA
CTATATCAACAACATGGAAATTGATTTTGGATTTGCAAGA