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- Construct 'CONS00000749'
Construct
ID
CONS00000749
Name
pCi-Nut1 -123/94 pVenus::EGFP
Min Promoter
Reporter
Comment
Construct containing the region upstream of Ci-Nut1 from -123bp to 94bp, this region is the minimal enhancer for Nut expression. This region was inserted into the promoter of Venus reporter gene.
Primers used for the construct : Forward primer Nut1UPSU-119/SalI 5-AACCGTCGACTGTCTAAATCCATGCGAAAC-3 and the reverse primer Nut1UPSL/BamHI 5-CGCGGATCCTCCAAAATCAATTTCCATG-3.
Show sequence
Length: 217
TTCACTGCGTTGTCTAAATCCATGCGAAACAACAAAATAAGTCGCAAGACATGCTGTGCG
TGTATTGTTTTTTTGAAACCCCGTCGCTTTGTGAAAATCTGGTTGATTATTTTTTCGTAC
CAGTTTTACAGTTTAAATACGACTGTGCTTCAGTTTTTGTTAGTATTGAGTTGTACACTA
TATCAACAACATGGAAATTGATTTTGGATTTGCAAGA