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CONSTRUCT

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  1. Clone 'AHC0AAA32YG12'
  2. Construct 'CONS00000699'

Construct

ID

CONS00000699

Name

pCi-Hox1 -836/553 delta -496/-477::LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

This construct contains the element 2, but the first conserved element was deleted. Construct named Ci-Hox1(del1)/LacZ in the paper. The deletion has no effect on the epidermal expression.
The sequence was found thanks to the primers used in the paper. The coordinates were based on the first exon on the KH transcript. The deleted sequence was replaced by "-".

This construct is testing Regulatory Region

REG00000732: Ci-Hox1 -836/553 delta -496/-477

Sequence

Show sequence

Length: 1,389

GTCGACGAACGATCATCATGCCAACATTTAAAATACAACTTTAGGTATTTTCCATTGGAT
TTAGAGCTTACAAAGACCAGATTGTGTTACCTAGTGTTCAACCATATTCAAATGCAATTA
AATGCACTGCCAACAGCGTGTTTATAAACACTATTCGGTGCTTCTATGGGATGCGTGAGC
AATCTATATGTGAAGTATAATAAATCTTCGTGTAGTAAAATTGTTGTCAATATAAATTTG
TAAACATTTTTTTTTTGGTTACGAATTTACCAAAACGTAATGTGGTTAAAGCGACTAAAA
TTTGCAGGAGATTTGCTATGTTTCTTATATTAAAATTGGA--------------------
TGACCGGACACTGGAAACAACAACAAAATGAGAAACGCGCCGAAAGCTCGAAGATAATAA
GCAAACAAACGATGAACTTTTCACCCTAGAGTGAAGACCGGACAAGTGCTCTTGTCCGAG
CGCGGACATTGCTGTTTTTACAAGATGAATAAAAGAAACAAAAAACTCTCGTGTGCGTGG
AGAAACAAGGAGAGAATAGAAACGAAAAAGTGTGAAAGAAATAGGGGAGCTTGCGTTTGT
ATAAAACGAGGTGCTGGACGGGTTAGAAACTTCTCTGCCACAGATTGTAAAAGGCAACAA
ACCATATTTAGTATGTTTCGCTTTGAAAGAAATAGTGTTTGCATTTTGGACCAGTGTTAT
ATTGTATTGCTTTATACTAGTAGGTAAAGAAAAAACTAAAAAATTACTATTACATCGATG
CAAACATTGGCTAAATATGAAGTTGGGGTGTTGCGTTTAATCTTTGCTAGTACAGTATTG
TGGTTTACTAGGTTGTTTAGACTTTAGGTAAAATTAAAGTTAAAACAAAAATTGTAGCAG
CAACACACGAAGGATCTGCACAGCTCGTTAATAGGTATGTGTAATTATTTTTACTGATAA
AATTAGCATTTAAGAGTAGCCGCTGCGGCATTGGTATTCTGTTTCTATTTTTATATTTAG
TCATTTTGATAGTAATTCAACATCAATTAAATTAACGTACCATTATTATTATTCCTACCA
ATGTTTACCTAAAATTCAAGCAGATACATTTGTAATTAACCATATAACGTTTATTATTCG
ATTTACACGCGTATTTGAAGGACTGAAAATGAAGTTTGCTTCAATTTAGGAATCTCATTG
TACGTCGATATAGCTTTGCGAATTTGGGTAAAAAGCGTTACACAACAACGGCTTATTAGA
ACCTTATTTACTGCCAAGACTATTAAATACTCTTTTGTTAGAAAGGTGTAAGATGAATTC
GTACATGAAATACCCGGTTCATGCTTCAAACTCAAATTCCTATACCAACCACATCACCCC
GCAAGGCCC

Reference(s)