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CONSTRUCT

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  1. Construct 'CONS00000669' > 
  2. Construct 'CONS00000668'

Construct

ID

CONS00000668

Name

pCi-Pitx -2751/-2696 repeated x2 Second Smad site mutated:CES2:LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

Minimal enhancer upstream of Pitx gene from -2751 to -2696, called D1(ab)(ab-Gmut) in the paper. The region named D1ab in the paper was repeated 2 times and the Smad site in the repeated region was deleted. This region can t drive expression in the neurohypophysis primordium.
The mutated sequence (Smad site) was replaced by "N". The sequence is based on the first codon on the KH transcript.

This construct is testing Regulatory Region

REG00000699: Ci-Pitx -2751/-2696 repeated x2 Second Smad site mutated

Sequence

Show sequence

Length: 110

AAACGCGACGACCTCCACGGATTAAGGTAATATTTCTTTTTATGTTGCCGCCCAGAAACG
CGACGACCTCCACGGATTAAGGTAATATTTCTTTTTATGTTNNNNCCCAG

Reference(s)

Show in situ data