- Construct 'CONS00000666'
Construct
ID
CONS00000666
Name
pCi-Pitx -2751/-2696 repeated x2 Second Otx site mutated:CES2:LacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
Minimal enhancer upstream of Pitx gene from -2751 to -2696, called D1(ab)(ab-Omut) in the paper. The region named D1ab in the paper was repeated 2 times and the Otx site in the repetition was mutated. This region can t drive expression in the neurohypophysis primordium.
The Otx site mutated was replaced by "N" in the DNA sequence.
REG00000697: Ci-Pitx -2751/-2696 repeated x2 Second Otx site mutated
AAACGCGACGACCTCCACGGATTAAGGTAATATTTCTTTTTATGTTGCCGCCCAGAAACG
CGACGACCTCCACGNNNNNAGGTAATATTTCTTTTTATGTTGCCGCCCAG