Login
Help

CONSTRUCT

Submit your Data

  1. Construct 'CONS00000666'

Construct

ID

CONS00000666

Name

pCi-Pitx -2751/-2696 repeated x2 Second Otx site mutated:CES2:LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

Minimal enhancer upstream of Pitx gene from -2751 to -2696, called D1(ab)(ab-Omut) in the paper. The region named D1ab in the paper was repeated 2 times and the Otx site in the repetition was mutated. This region can t drive expression in the neurohypophysis primordium.
The Otx site mutated was replaced by "N" in the DNA sequence.

This construct is testing Regulatory Region

REG00000697: Ci-Pitx -2751/-2696 repeated x2 Second Otx site mutated

Sequence

Show sequence

Length: 110

AAACGCGACGACCTCCACGGATTAAGGTAATATTTCTTTTTATGTTGCCGCCCAGAAACG
CGACGACCTCCACGNNNNNAGGTAATATTTCTTTTTATGTTGCCGCCCAG

Reference(s)