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- Construct 'CONS00000654'
Construct
ID
CONS00000654
Name
pCi-Pitx -2735/-2540 First Forkhead site mutated:CES2:LacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
Minimal enhancer upstream of Pitx gene from -2735 to -2540, called D1bcde in the paper. The Pax binding site is absent. The first Forkhead regulatory motif was mutated (TAATATTTCTTT => TAATATGGCGGT). The mutation (m1 in the paper) affects the expression in the neurohypophysis primordium.
KH transcript model was taken as reference for the region s coordinates.
The sequence is based on the first codon on the KH transcript.
REG00000687: Ci-Pitx -2735/-2540 First Forkhead site mutated
ACGGATTAAGGTAATATGGCGGTTTATGTTGCCGCCCAGTCGGTTCCTGACAATACACTG
ACAGGTAGTGCGAATAAAAGGCGCTCGTGGATGTAAAAAAACACTAATAATCTTCAGTAA
TTTCCGTGCCTTTCCTATGGGCGAAAACTCCCGCCGTGTGAAACGACTTCAAAAGGCGGA
CAGTCGCCGCCAAGG