- Construct 'CONS00000641'
Construct
ID
CONS00000641
Name
pCi-RhoD 565/989 Ets1/2-1-2 mutated pFOG::LacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
the two Ets1/2 sites were mutated and the RhoD expression is lost in B7.5 lineage.
Be careful, coordinates in the paper are different from ANISEED coordinates. We took the KH transcript model as reference.
Coordinates in the paper : +498/+922
The two Ets-1/2 sites mutated (GGA-> AGA)were remplaced by "-".
Transcription start site is based on the start of the first exon of the KH transcript model.
ACTAAATTTAATTAGCAACCCGCTGTTGTTCAAAGGTGTACACGCCCACGGTGTTTGAGA
ATTACAGCAGAGAGGTGGAATTAAATCGAAAGAAGGCGGTGCTAACATTGTGGGATACGG
CGGGTATATATTAGTACATACGTGTTTATGCAAGACATCGTAGGCTTGTTGACTTAGTTT
CGGTTAGCA------TGTAGTCTATGTTCTACGTGGGTTATGTTCTTGTCG------TGG
GATTAGGGCTAAATTCAGCCTATTATTACCACAACATTCAGACTGCTTTTGTAGGTCCAA
ATGTAACATATGACTAGTGTGTTCTGATGTGTGTTTATAATACAATAAACTAGCGCAAAC
TGTTTGTCCCATTATGTTTAGTCATATAGGTCATATGAGAGTAAATGAAGTGTAAACCAA
ATGT