Login
Help

CONSTRUCT

Submit your Data

  1. Construct 'CONS00000641'

Construct

ID

CONS00000641

Name

pCi-RhoD 565/989 Ets1/2-1-2 mutated pFOG::LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

the two Ets1/2 sites were mutated and the RhoD expression is lost in B7.5 lineage.
Be careful, coordinates in the paper are different from ANISEED coordinates. We took the KH transcript model as reference.
Coordinates in the paper : +498/+922
The two Ets-1/2 sites mutated (GGA-> AGA)were remplaced by "-".
Transcription start site is based on the start of the first exon of the KH transcript model.

This construct is testing Regulatory Region

REG00000679: Ci-RhoD 565/989 Ets1/2-1-2 mutated

Sequence

Show sequence

Length: 424

ACTAAATTTAATTAGCAACCCGCTGTTGTTCAAAGGTGTACACGCCCACGGTGTTTGAGA
ATTACAGCAGAGAGGTGGAATTAAATCGAAAGAAGGCGGTGCTAACATTGTGGGATACGG
CGGGTATATATTAGTACATACGTGTTTATGCAAGACATCGTAGGCTTGTTGACTTAGTTT
CGGTTAGCA------TGTAGTCTATGTTCTACGTGGGTTATGTTCTTGTCG------TGG
GATTAGGGCTAAATTCAGCCTATTATTACCACAACATTCAGACTGCTTTTGTAGGTCCAA
ATGTAACATATGACTAGTGTGTTCTGATGTGTGTTTATAATACAATAAACTAGCGCAAAC
TGTTTGTCCCATTATGTTTAGTCATATAGGTCATATGAGAGTAAATGAAGTGTAAACCAA
ATGT

Reference(s)