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- Construct 'CONS00000857' >
- Construct 'CONS00000670'
Construct
ID
CONS00000670
Name
pCi-Pitx -2751/-2696 repeated x2/50bp spacer:CES2:LacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
Minimal enhancer upstream of Pitx gene from -2751 to -2696, called D1(ab)-50-(ab) in the paper. The region named D1ab in the paper was repeat once, and between the two fragments there is a spacer region of 50bp. This region can t drive expression in the neurohypophysis primordium.
The spacer region added is represented by "N". The sequence is based on the first codon on the KH transcript.
AAACGCGACGACCTCCACGGATTAAGGTAATATTTCTTTTTATGTTGCCGCCCAGNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAACGCGACGACCTC
CACGGATTAAGGTAATATTTCTTTTTATGTTGCCGCCCAG