- Construct 'CONS00000671'
Construct
ID
CONS00000671
Name
pCi-Pitx -2751/-2696 repeated x2/75bp spacer:CES2:LacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
Minimal enhancer upstream of Pitx gene from -2751 to -2696, called D1(ab)-75-(ab) in the paper. The region named D1ab in the paper was repeat once, and between the two fragments there is a spacer region of 75bp. This region can t drive expression in the neurohypophysis primordium.
The spacer region added was represented by "N". The sequence is based on the first codon on the KH transcript.
Show sequence
Length: 185
AAACGCGACGACCTCCACGGATTAAGGTAATATTTCTTTTTATGTTGCCGCCCAGNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNAAACGCGACGACCTCCACGGATTAAGGTAATATTTCTTTTTATGTTGCCG
CCCAG
